黑腹果蝇和暗果蝇之间固定的倒位断点的分子特征分析。
Molecular characterization of the breakpoints of an inversion fixed between Drosophila melanogaster and D. subobscura.
作者信息
Cirera S, Martín-Campos J M, Segarra C, Aguadé M
机构信息
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain.
出版信息
Genetics. 1995 Jan;139(1):321-6. doi: 10.1093/genetics/139.1.321.
Abstract
The two breakpoints of a chromosomal inversion fixed since the split of Drosophila melanogaster and D. subobscura lineages have been isolated and sequenced in both species. The regions spanning the breakpoints initially were identified by the presence of two signals after interspecific in situ hybridization on polytene chromosomes. Interspecific comparison of the sequenced regions allowed us to delineate the location of the breakpoints. Close to one of these breakpoints a new transcription unit (bcn92) has been identified in both species. The inversion fixed between D. melanogaster and D. subobscura does not seem to have broken any transcription unit. Neither complete nor defective transposable elements were found in the regions encompassing the breakpoints. Short thymine-rich sequences (30-50 bp long) have been found bordering the breakpoint regions. Although alternating Pur-Pyr sequences were detected, these putative target sites for topoisomerase II were not differentially clustered in the breakpoints.
摘要
自黑腹果蝇和暗果蝇谱系分化以来固定的染色体倒位的两个断点已在两个物种中分离并测序。最初，通过在多线染色体上进行种间原位杂交后出现的两个信号来识别跨越断点的区域。对测序区域的种间比较使我们能够确定断点的位置。在这两个物种中，已在其中一个断点附近鉴定出一个新的转录单位（bcn92）。黑腹果蝇和暗果蝇之间固定的倒位似乎没有破坏任何转录单位。在包含断点的区域中未发现完整或有缺陷的转座元件。已发现富含胸腺嘧啶的短序列（30 - 50个碱基对长）毗邻断点区域。尽管检测到嘌呤 - 嘧啶交替序列，但这些假定的拓扑异构酶II靶位点在断点处没有差异聚集。
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本文引用的文献
1
Nucleotide divergence of the rp49 gene region between Drosophila melanogaster and two species of the Obscura group of Drosophila.黑腹果蝇与果蝇暗果蝇组的两个物种之间rp49基因区域的核苷酸差异。
J Mol Evol. 1993 Mar;36(3):243-8. doi: 10.1007/BF00160479.
2
Isolation and analysis of the breakpoint sequences of chromosome inversion In(3L)Payne in Drosophila melanogaster.黑腹果蝇3号染色体左臂Payne倒位断点序列的分离与分析
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3132-6. doi: 10.1073/pnas.91.8.3132.
3
Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.DNA测序鸟枪法产生的凝胶读数数据的计算机处理自动化。
Nucleic Acids Res. 1982 Aug 11;10(15):4731-51. doi: 10.1093/nar/10.15.4731.
4
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
5
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.用核酸外切酶III进行单向消化可为DNA测序创建靶向断点。
Gene. 1984 Jun;28(3):351-9. doi: 10.1016/0378-1119(84)90153-7.
6
Formation of chromosome rearrangements by P factors in Drosophila.果蝇中P因子导致染色体重排的形成。
Genetics. 1984 Aug;107(4):657-78. doi: 10.1093/genetics/107.4.657.
7
Karyotypic stability and speciation in Hawaiian Drosophila.夏威夷果蝇的核型稳定性与物种形成
Proc Natl Acad Sci U S A. 1967 May;57(5):1280-5. doi: 10.1073/pnas.57.5.1280.
8
Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions.估计同义与非同义核苷酸替换数目的简单方法。
Mol Biol Evol. 1986 Sep;3(5):418-26. doi: 10.1093/oxfordjournals.molbev.a040410.
9
Nucleotide sequence comparison of the rp49 gene region between Drosophila subobscura and D. melanogaster.果蝇亚暗果蝇和黑腹果蝇rp49基因区域的核苷酸序列比较。
Mol Biol Evol. 1988 Jul;5(4):433-41. doi: 10.1093/oxfordjournals.molbev.a040502.
10
The cloning of the Bar region and the B breakpoint in Drosophila melanogaster: evidence for a transposon-induced rearrangement.果蝇中Bar区域和B断点的克隆：转座子诱导重排的证据
Genetics. 1989 Aug;122(4):881-90. doi: 10.1093/genetics/122.4.881.
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