果蝇中Bar区域和B断点的克隆：转座子诱导重排的证据
The cloning of the Bar region and the B breakpoint in Drosophila melanogaster: evidence for a transposon-induced rearrangement.
作者信息
Tsubota S I, Rosenberg D, Szostak H, Rubin D, Schedl P
机构信息
Department of Biology, University of Michigan, Ann Arbor 48109.
出版信息
Genetics. 1989 Aug;122(4):881-90. doi: 10.1093/genetics/122.4.881.
Abstract
We have cloned the B breakpoint in Drosophila melanogaster using DNA from a P-M-induced revertant of B, which has a P element inserted at the B breakpoint. The analysis of the B DNA reveals that there is a transposable element, B104, right at the breakpoint. This suggests that this element may have been involved in the generation of the B breakpoint and the associated tandem duplication. One possible mechanism to generate the B duplication is a recombination event between two B104 elements, one at 16A1 and the other at 16A7. DNA sequencing data of the junctions of the B104 element support this model. Four partial revertants of B are the result of insertions of transposable elements very close to the B breakpoint. This supports the hypothesis that the breakpoint is the cause of the B mutation. The clones from B were used to isolate wild-type clones from 16A1, the location of the Bar gene. Four rearrangement breakpoints associated with various Bar mutations map within a 37-kb region, suggesting that the Bar gene is very large.
摘要
我们利用来自P-M诱导的B回复体的DNA克隆了黑腹果蝇中的B断点，该回复体在B断点处插入了一个P因子。对B DNA的分析表明，在断点处正好有一个转座元件B104。这表明该元件可能参与了B断点及相关串联重复的产生。产生B重复的一种可能机制是两个B104元件之间的重组事件，一个在16A1，另一个在16A7。B104元件连接处的DNA测序数据支持这一模型。B的四个部分回复体是转座元件插入到非常接近B断点的位置的结果。这支持了断点是B突变原因的假说。来自B的克隆用于从Bar基因所在的16A1分离野生型克隆。与各种Bar突变相关的四个重排断点定位在一个37 kb的区域内，这表明Bar基因非常大。
相似文献
1
The cloning of the Bar region and the B breakpoint in Drosophila melanogaster: evidence for a transposon-induced rearrangement.果蝇中Bar区域和B断点的克隆：转座子诱导重排的证据
Genetics. 1989 Aug;122(4):881-90. doi: 10.1093/genetics/122.4.881.
2
Unequal crossing-over within the B duplication of Drosophila melanogaster: a molecular analysis.果蝇黑腹果蝇B重复序列内的不等交换：分子分析
Genet Res. 1991 Apr;57(2):105-11. doi: 10.1017/s0016672300029165.
3
The identification of the Bs breakpoint and of two possible Bar genes.Bs断点以及两个可能的Bar基因的鉴定。
Mol Gen Genet. 1992 May;233(1-2):106-12. doi: 10.1007/BF00587567.
4
DNA sequence of the Doc retroposon in the white-one mutant of Drosophila melanogaster and of secondary insertions in the phenotypically altered derivatives white-honey and white-eosin.黑腹果蝇白色-1突变体中Doc反转座子的DNA序列以及表型改变的衍生物白色-蜜色和白色-曙红中的二次插入序列。
Mol Gen Genet. 1991 Jan;225(1):17-24. doi: 10.1007/BF00282637.
5
One side of a deletion breakpoint from the Drosophila melanogaster genome contains a transposable element.来自黑腹果蝇基因组的一个缺失断点的一侧含有一个转座元件。
Gene. 1983 Jan-Feb;21(1-2):87-94. doi: 10.1016/0378-1119(83)90150-6.
6
The isolation of the acid phosphatase-1 gene of Drosophila melanogaster and a chromosomal breakpoint inducing its position effect variegation.黑腹果蝇酸性磷酸酶-1基因的分离以及诱导其位置效应斑驳的一个染色体断点
Mol Gen Genet. 1990 Oct;224(1):49-56. doi: 10.1007/BF00259450.
7
Excision of copia element in a revertant of the white-apricot mutation of Drosophila melanogaster leaves behind one long-terminal repeat.在黑腹果蝇白眼杏色突变体的回复体中，copia元件的切除留下了一个长末端重复序列。
Mol Gen Genet. 1985;199(1):1-6. doi: 10.1007/BF00327501.
8
The X element, a novel LINE transposable element from Drosophila melanogaster.X元素，一种来自黑腹果蝇的新型LINE转座元件。
Mol Genet Genomics. 2001 May;265(3):489-96. doi: 10.1007/s004380000437.
9
The molecular organization of the Star/asteroid region, a region necessary for proper eye development in Drosophila melanogaster.星状体/小行星区域的分子组织，这是黑腹果蝇正常眼睛发育所必需的一个区域。
Genome. 1993 Apr;36(2):356-66. doi: 10.1139/g93-049.
10
Cloning of the esterase-5 locus from Drosophila pseudoobscura and comparison with its homologue in D. melanogaster.拟暗果蝇酯酶-5基因座的克隆及其与黑腹果蝇同源基因的比较。
Mol Biol Evol. 1990 Nov;7(6):525-46. doi: 10.1093/oxfordjournals.molbev.a040624.
引用本文的文献
1
The landscape of submicroscopic structural variants at the gene cluster on Xq28 underlying blue cone monochromacy.Xq28 基因簇上导致蓝色锥细胞单色视的亚微观结构变异景观。
Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2115538119. doi: 10.1073/pnas.2115538119. Epub 2022 Jun 27.
2
The Draft Genome of Kochia scoparia and the Mechanism of Glyphosate Resistance via Transposon-Mediated EPSPS Tandem Gene Duplication.多粘菌素抗性基因结构与机制的研究进展
多粘菌素是一种环状多肤类抗生素，自 20 世纪 50 年代被发现以来，一直作为兽医临床上治疗革兰氏阴性菌感染的最后一道防线。然而，近年来，多粘菌素抗性基因在临床和环境中的出现和传播引起了人们的广泛关注。本文综述了多粘菌素抗性基因的结构、分类、传播机制以及在临床和环境中的分布和流行情况，并探讨了多粘菌素抗性基因对人类健康和环境安全的潜在威胁。最后，提出了一些应对多粘菌素抗性基因的策略，包括加强抗生素的管理和规范使用、加强对多粘菌素抗性基因的监测和研究以及开发新型抗生素等，以应对多粘菌素抗性基因的挑战。
Genome Biol Evol. 2019 Oct 1;11(10):2927-2940. doi: 10.1093/gbe/evz198.
3
Maize Transposon Storm Kicks up a .玉米转座子风暴掀起了一场…… （原文似乎不完整）
Genetics. 2017 May;206(1):87-89. doi: 10.1534/genetics.116.199638.
4
Alfred Sturtevant Walks into a Bar: Gene Dosage, Gene Position, and Unequal Crossing Over in Drosophila.阿尔弗雷德·斯特蒂文特走进一家酒吧：果蝇中的基因剂量、基因位置与不等交换
Genetics. 2016 Nov;204(3):833-835. doi: 10.1534/genetics.116.195891.
5
Rare recombination events generate sequence diversity among balancer chromosomes in Drosophila melanogaster.罕见的重组事件在黑腹果蝇的平衡染色体间产生序列多样性。
Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):E1352-61. doi: 10.1073/pnas.1601232113. Epub 2016 Feb 22.
6
Mechanisms of gene duplication and amplification.基因复制与扩增的机制。
Cold Spring Harb Perspect Biol. 2015 Feb 2;7(2):a016592. doi: 10.1101/cshperspect.a016592.
7
Multiple pathways of duplication formation with and without recombination (RecA) in Salmonella enterica.沙门氏菌中有无重组（RecA）的多种重复形成途径。
Genetics. 2012 Oct;192(2):397-415. doi: 10.1534/genetics.112.142570. Epub 2012 Aug 3.
8
The amplification and evolution of orthologous 22-kDa α-prolamin tandemly arrayed genes in coix, sorghum and maize genomes.同源 22kDaα-醇溶蛋白串联排列基因在薏苡、高粱和玉米基因组中的扩增和进化。
Plant Mol Biol. 2010 Dec;74(6):631-43. doi: 10.1007/s11103-010-9705-5. Epub 2010 Oct 12.
9
2010: A century of Drosophila genetics through the prism of the white gene.2010：从白眼基因看果蝇遗传学的一个世纪
Genetics. 2010 Jan;184(1):3-7. doi: 10.1534/genetics.109.110015.
10
Unequal sister chromatid and homolog recombination at a tandem duplication of the A1 locus in maize.玉米A1位点串联重复处的不等姐妹染色单体和同源重组。
Genetics. 2006 Aug;173(4):2211-26. doi: 10.1534/genetics.105.052712. Epub 2006 Jun 4.
本文引用的文献
1
THE BAR "GENE" A DUPLICATION.“BAR”基因重复。
Science. 1936 Feb 28;83(2148):210-1. doi: 10.1126/science.83.2148.210.
2
Studies on Some Position Pseudoalleles at the White Region in Drosophila Melanogaster.黑腹果蝇白色区域某些位置拟等位基因的研究。
Genetics. 1959 Jan;44(1):34-42. doi: 10.1093/genetics/44.1.34.
3
Bar Eye in Drosophila Melanogaster: A Cytological Analysis of Some Mutations and Reverse Mutations.黑腹果蝇的棒眼：一些突变和回复突变的细胞学分析
Genetics. 1943 Mar;28(2):97-107. doi: 10.1093/genetics/28.2.97.
4
The Effects of Unequal Crossing over at the Bar Locus in Drosophila.果蝇中棒眼基因座不等交换的影响
Genetics. 1925 Mar;10(2):117-47. doi: 10.1093/genetics/10.2.117.
5
Frequent Imprecise Excision among Reversions of a P Element-Caused Lethal Mutation in Drosophila.果蝇中 P 元素引起的致死突变回复体中的频繁不精确切除。
Genetics. 1984 Jun;107(2):279-94. doi: 10.1093/genetics/107.2.279.
6
Unequal crossing-over associated with asymmetrical synapsis between nomadic elements in the Drosophila melanogaster genome.果蝇基因组中游牧元件之间不对称联会导致不等交换。
Proc Natl Acad Sci U S A. 1983 Aug;80(16):5017-21. doi: 10.1073/pnas.80.16.5017.
7
Molecular analysis of the yellow locus of Drosophila.果蝇黄色基因座的分子分析。
EMBO J. 1986 Dec 20;5(13):3597-605. doi: 10.1002/j.1460-2075.1986.tb04688.x.
8
Putative non-reciprocal crossing over in Drosophila melanogaster.黑腹果蝇中假定的非相互交换
Z Vererbungsl. 1959;90:375-84. doi: 10.1007/BF00888812.
9
Formation of duplication-deficiency products by asymmetrical exchange within a complex locus of Drosophila melanogaster.通过黑腹果蝇复杂基因座内的不对称交换形成重复-缺失产物。
Proc Natl Acad Sci U S A. 1961 Apr 15;47(4):545-50. doi: 10.1073/pnas.47.4.545.
10
Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome.果蝇基因组中P转座因子的结构及其插入和切除位点。
Cell. 1983 Aug;34(1):25-35. doi: 10.1016/0092-8674(83)90133-2.
Zotero 插件