Moore R Y, Speh J C, Card J P
Department of Psychiatry, University of Pittsburgh, Pennsylvania 15261, USA.
J Comp Neurol. 1995 Feb 13;352(3):351-66. doi: 10.1002/cne.903520304.
The retinal ganglion cells giving rise to retinohypothalamic projections in the rat were identified using retrograde transport of horseradish peroxidase (HRP) or FluoroGold injected into the suprachiasmatic nucleus (SCN), and using transneuronal transport of the Bartha strain of the swine herpesvirus (PRV-Bartha). When PRV-Bartha is injected into one eye, it is taken up by retinal ganglion cells, replicated, transported to axon terminals in the SCN, and released. There the virus may take one, or both, of two paths to retinal ganglion cells in the contralateral eye: 1) uptake by SCN neurons, replication, and release from the neurons with uptake and retrograde transport in retinal afferents originating in the contralateral retina; 2) transneuronal passage through axo-axonic appositions between retinal afferents in the SCN with subsequent retrograde transport of virus to the contralateral retina. The ganglion cells thus labeled are a homogeneous population of small neurons (mean diameter, 12.8 +/- 2.2 microns and mean area, 81.8 +/- 21.8 microns 2) with sparsely branching dendrites that are widely distributed over the retina. This population is best identified when virus labeling of retinal projections in areas beyond the hypothalamus is eliminated by lateral geniculate lesions that transect the optic tract at its entry into the geniculate complex. The same population is labeled with retrograde tracers but, with both HRP and FluoroGold, other ganglion cells are labeled, presumably from uptake by fibers of passage, indicating that the virus is a more reliable marker for ganglion cells giving rise to retinohypothalamic projections. The ganglion cells identified correspond to a subset of type III, or W, cells.
利用辣根过氧化物酶（HRP）或注入视交叉上核（SCN）的荧光金的逆行运输，以及猪疱疹病毒（PRV-Bartha）Bartha株的跨神经元运输，鉴定了大鼠中产生视网膜下丘脑投射的视网膜神经节细胞。当将PRV-Bartha注入一只眼睛时，它会被视网膜神经节细胞摄取、复制，运输到视交叉上核的轴突终末并释放。在那里，病毒可能通过两条路径中的一条或两条到达对侧眼中的视网膜神经节细胞：1）被视交叉上核神经元摄取、复制，然后从神经元释放，被源自对侧视网膜的视网膜传入纤维摄取并逆行运输；2）通过视交叉上核中视网膜传入纤维之间的轴-轴突触进行跨神经元传递，随后病毒逆行运输到对侧视网膜。这样标记的神经节细胞是一群均匀的小神经元（平均直径为12.8±2.2微米，平均面积为81.8±21.8平方微米），其树突分支稀疏，广泛分布于视网膜上。当通过在视束进入外侧膝状体复合体处横断视束的外侧膝状体损伤消除下丘脑以外区域的视网膜投射的病毒标记时，这群细胞最容易被识别。用逆行示踪剂也标记了相同的细胞群，但使用HRP和荧光金时，其他神经节细胞也被标记，推测是由于过路纤维的摄取，这表明病毒是产生视网膜下丘脑投射的神经节细胞的更可靠标记。所鉴定的神经节细胞对应于III型或W型细胞的一个子集。
