将人巨细胞病毒注射到兔眼后未出现感染性视网膜炎。

Absence of infectious retinitis after injection of human cytomegalovirus into rabbit eyes.

作者信息

Tatebayashi M, Neyts J, Besen G, Flores-Aguilar M, Smith I L, Wiley C A, Spector S A, Bergeron-Lynn G, Maudgal P C, De Clercq E

机构信息

Department of Ophthalmology, School of Medicine, University of California, San Diego, La Jolla 92093-0946.

出版信息

J Infect Dis. 1995 Apr;171(4):782-7. doi: 10.1093/infdis/171.4.782.

DOI:10.1093/infdis/171.4.782

PMID:7706803

Abstract

The rabbit model of human cytomegalovirus (HCMV) retinitis was evaluated by preretinal and intravitreal injection of HCMV into rabbit eyes. Ocular disease was evaluated by indirect ophthalmoscopy, histopathology, and immunohistochemistry. Vitritis, optic nerve congestion, multiple small white infiltrates in the cortical vitreous or on the retinal surface, and retinal detachments were seen. Histopathologic examination showed inflammatory cell infiltration in the preretinal vitreous, optic nerve, and transiently in the superficial inner retina. Retinal structure was preserved except for changes in areas of retinal detachment. No necrosis or destruction of the retina was seen. Immunohistochemistry showed no evidence of cytomegalovirus infection. Inoculation of culture medium containing fetal calf serum caused a similar reaction. It is concluded that vitreous and retinal inoculation of HCMV in the rabbit eye caused nonspecific inflammation without evidence of infection, so this is not a suitable model for HCMV retinitis.

摘要

通过将人巨细胞病毒（HCMV）视网膜下注射和玻璃体内注射到兔眼中，对HCMV视网膜炎的兔模型进行了评估。通过间接检眼镜检查、组织病理学和免疫组织化学评估眼部疾病。观察到玻璃体炎、视神经充血、皮质玻璃体或视网膜表面有多个小白斑浸润以及视网膜脱离。组织病理学检查显示视网膜前玻璃体、视神经有炎性细胞浸润，视网膜内表面浅层有短暂性炎性细胞浸润。除视网膜脱离区域有变化外，视网膜结构得以保留。未观察到视网膜坏死或破坏。免疫组织化学未显示巨细胞病毒感染的证据。接种含胎牛血清的培养基也引起了类似反应。得出的结论是，在兔眼中玻璃体和视网膜接种HCMV会引起非特异性炎症，且无感染证据，因此这不是HCMV视网膜炎的合适模型。