Attardi B, Ruoslahti E
Steroids. 1977 Nov;30(5):711-6. doi: 10.1016/0039-128x(77)90060-5.
The hypothesis that alphafetoprotein (AFP) could be a consitituent of the estradiol receptor in the uterus and brain of the immature mouse has been tested. Cytosols prepared from 2 week-old mice were depleted of AFP by immunoadsorption with a Sepharose-coupled anti-mouse AFP anti-serum. Aliquots of these cytosols containing nondetectable levels of AFP by RIA were then brought to 0.4 M KCl to convert 8 S estradiol receptors to the 4-5 S forms, reacted either with anti-AFP or with a control anti-IgG immunoadsorbent, labeled with 3H-estradiol, and centrifuged on glycerol gradients. There was no, or very little, loss of estradiol receptors in the experimental as compared to the control cytosols indicating that AFP is not a constituent of the 8 S estradiol receptor.
甲胎蛋白（AFP）可能是未成熟小鼠子宫和大脑中雌二醇受体的组成成分这一假说已得到验证。用琼脂糖偶联的抗小鼠AFP抗血清通过免疫吸附法去除2周龄小鼠制备的胞质溶胶中的AFP。然后将这些通过放射免疫分析检测不到AFP水平的胞质溶胶等分试样加入到0.4M KCl中，使8S雌二醇受体转化为4 - 5S形式，与抗AFP或对照抗IgG免疫吸附剂反应，用³H - 雌二醇标记，并在甘油梯度上离心。与对照胞质溶胶相比，实验胞质溶胶中雌二醇受体没有或仅有极少损失，这表明AFP不是8S雌二醇受体的组成成分。
