Putnam J L, Scott T W
Department of Entomology, University of Maryland, College Park 20742, USA.
J Parasitol. 1995 Apr;81(2):170-4.
This study was designed to determine if transmission rates for dengue-2 virus by Aedes aegypti are altered by mosquitos probing a host for blood or imbibing blood prior to attempting transmission. Aedes aegypti is known to contact multiple hosts during each egg-laying cycle and multiple host contacts might diminish the amount of virus in infected mosquito's salivary glands or ducts and render them functionally uninfective. Probing a host 5, 10, or 20 consecutive times did not significantly alter the infectivity of parenterally infected mosquitoes. However, orally infected Ae. aegypti that probed 20 times transmitted dengue viruses at a significantly higher rate than controls. Infectivity of orally infected Ae. aegypti was unaffected by blood feeding. Our data suggest that (1) dengue virus-infected Ae. aegypti remain infective regardless of their probing or engorging history, and (2) once Ae. aegypti become infective they are extremely efficient disseminators of dengue virus.
本研究旨在确定埃及伊蚊传播登革2型病毒的速率是否会因蚊子在尝试传播之前探测宿主取血或吸食血液而发生改变。已知埃及伊蚊在每个产卵周期会接触多个宿主，多次接触宿主可能会减少受感染蚊子唾液腺或导管中的病毒量，并使其失去功能传染性。连续5次、10次或20次探测宿主并不会显著改变经肠感染蚊子的传染性。然而，经口感染的埃及伊蚊探测20次后传播登革病毒的速率显著高于对照组。经口感染的埃及伊蚊的传染性不受取食血液的影响。我们的数据表明：（1）感染登革病毒的埃及伊蚊无论其探测或饱食历史如何，仍具有传染性；（2）一旦埃及伊蚊具有传染性，它们就是登革病毒极其高效的传播者。
