File S
Department of Biology, University of Puerto Rico, San Juan 00931-3360, USA.
J Parasitol. 1995 Apr;81(2):234-8.
The interaction of schistosome eggs with venular endothelium was studied using primary cultures of human umbilical vein endothelial cells. Freshly oviposited and embryonated eggs of Schistosoma mansoni, Schistosoma japonicum, and Schistosoma haematobium were used. The cultures were evaluated by light, scanning, and transmission electron microscopy. Endothelial cell monolayers were found to retain eggs by actively migrating over those that came to lie on top of them. The monolayers then reestablished confluency and their lumenal polarity. Eggs deposited directly by adult worms elicited a more rapid and complete response than embryonated eggs isolated from the liver tissues of infected rodents or latex beads. Cell migration was shown to be more complete in the presence of sera than in serum-free media. It is concluded from these observations that eggs can be passively transferred to the perivenular space by the nonspecific response of endothelial cells.
利用人脐静脉内皮细胞原代培养物研究了血吸虫卵与小静脉内皮的相互作用。使用了曼氏血吸虫、日本血吸虫和埃及血吸虫新产出的及已孵育的虫卵。通过光学显微镜、扫描电子显微镜和透射电子显微镜对培养物进行评估。发现内皮细胞单层通过主动迁移覆盖在其上方的虫卵来滞留虫卵。然后单层重新建立汇合状态及其管腔极性。成虫直接产下的虫卵比从感染啮齿动物肝脏组织中分离出的已孵育虫卵或乳胶珠引发的反应更快且更完全。与无血清培养基相比，在有血清存在的情况下细胞迁移更完全。从这些观察结果得出结论，虫卵可通过内皮细胞的非特异性反应被动转移至静脉周围间隙。
