Griveau J F, Dumont E, Renard P, Callegari J P, Le Lannou D
Cecos de l'Ouest, Rennes, France.
J Reprod Fertil. 1995 Jan;103(1):17-26. doi: 10.1530/jrf.0.1030017.
The reactive oxygen species, hydrogen peroxide (H2O2) and superoxide anion (O2o-), were generated with a xanthine-xanthine oxidase system and their effect on human sperm function was studied. The action of reactive oxygen species on selected human spermatozoa resulted in a decreased capacity for ionophore-induced acrosome reaction, a decrease in sperm motility, an increase in the concentration of lipid hydroperoxides and a loss of membrane polyunsaturated fatty acids. H2O2 was the key intermediate of the deleterious effects exerted by the xanthine and xanthine oxidase. Among these parameters, the acrosome reaction appeared most susceptible to the reactive oxygen species generated by the xanthine-xanthine oxidase system, and was decreased without sperm motility being affected. Treatment with H2O2 was shown to inactivate several enzymatic activities involved in the antioxidant defence of spermatozoa: glutathione peroxidase, superoxide dismutase and glucose-6-phosphate dehydrogenase. H2O2 and O2o- were shown to be involved in the lipid alterations triggered by the xanthine-xanthine oxidase system. Singlet oxygen is proposed to intervene in the lipoperoxidation process. The inefficacy of mannitol in protecting spermatozoa suggests that hydroxyl radicals were not produced in the extracellular medium.
采用黄嘌呤 - 黄嘌呤氧化酶系统生成活性氧物种、过氧化氢（H2O2）和超氧阴离子（O2o-），并研究它们对人类精子功能的影响。活性氧物种对选定的人类精子的作用导致离子载体诱导的顶体反应能力下降、精子活力降低、脂质氢过氧化物浓度增加以及膜多不饱和脂肪酸的损失。H2O2是黄嘌呤和黄嘌呤氧化酶产生有害作用的关键中间体。在这些参数中，顶体反应似乎对黄嘌呤 - 黄嘌呤氧化酶系统产生的活性氧物种最为敏感，并且在不影响精子活力的情况下降低。结果表明，用H2O2处理会使参与精子抗氧化防御的几种酶活性失活：谷胱甘肽过氧化物酶、超氧化物歧化酶和葡萄糖 - 6 - 磷酸脱氢酶。H2O2和O2o-被证明参与了由黄嘌呤 - 黄嘌呤氧化酶系统引发的脂质改变。单线态氧被认为参与了脂质过氧化过程。甘露醇对精子的保护无效表明细胞外介质中未产生羟基自由基。
