Cane P A, Pringle C R
Department of Biological Sciences, University of Warwick Coventry, United Kingdom.
J Virol. 1995 May;69(5):2918-25. doi: 10.1128/JVI.69.5.2918-2925.1995.
The variability of the attachment (G) proteins of 48 subgroup A isolates of respiratory syncytial virus (RSV) isolated over 38 years has been examined. Nucleotide sequences of two variable regions of the G protein genes were determined following amplification by PCR. The isolates showed temporal rather than geographical clustering, and there was evidence for progressive accumulation of amino acid changes at an average rate of approximately 0.25% per year estimated over the entire protein. The cocirculation of lineages of RSV at present appears to be the result of a process of evolution and survival of particular genotypes and the extinction of others. Analysis of reactivity of the isolates with monoclonal antibodies showed that their antigenic profiles closely paralleled their relatedness by nucleotide sequence, suggesting that antigenic drift due to immune selection may be occurring.
对38年间分离出的48株呼吸道合胞病毒（RSV）A亚组毒株的附着（G）蛋白变异性进行了研究。通过聚合酶链反应（PCR）扩增后，测定了G蛋白基因两个可变区的核苷酸序列。这些毒株呈现出时间上的聚类而非地理上的聚类，并且有证据表明，在整个蛋白质上估计氨基酸变化以每年约0.25%的平均速率进行渐进积累。目前RSV谱系的共同流行似乎是特定基因型进化和存活以及其他基因型灭绝过程的结果。对这些毒株与单克隆抗体反应性的分析表明，它们的抗原谱与其核苷酸序列的相关性密切平行，这表明可能正在发生由免疫选择导致的抗原漂移。
