Bergman H, Nagy J I, Granholm A C
Department of Cell Biology, University of Linköping, Sweden.
Neuroscience. 1995 Jan;64(1):61-70. doi: 10.1016/0306-4522(94)00371-b.
Investigations were undertaken to determine whether fetal histaminergic neurons in the tuberomammillary nucleus of the posterior hypothalamus survive intracranial transplantation to adult hosts. Two methods of transplantation were utilized. Grafts were placed either into the delayed cavity of a fimbria-fornix lesion or directly into the hippocampus using stereotaxic techniques. The tissue was taken from rat fetuses at embryonic days 16-17 and grafted into adult rats of either the Sprague-Dawley or the Fischer 344 strain. Routine histology and immunohistochemistry were used to evaluate the grafts. All transplants to Sprague-Dawley rats showed signs of rejection, while no signs of rejection were seen in any of the Fischer 344 rats. Transplants placed directly into the delayed fimbria-fornix cavity did not grow as well or contain as many surviving neurons as the intraparenchymal grafts. The largest number of surviving histamine-positive neurons was obtained with grafts of posterolateral blocks of hypothalamus from fetal day 17 placed directly into the CA1 region of the rostral hippocampal formation of Fischer 344 hosts. Histamine-immunoreactive cell bodies with neuritic outgrowth were found in all Fischer 344 rats that received hypothalamic grafts. Cell bodies exhibited histamine immunoreactivity evenly throughout the cytoplasm and had morphological characteristics resembling histaminergic neurons in situ. Axonal outgrowth extended throughout the grafted hypothalamic tissue, and was sometimes seen in the host hippocampal tissue as well. It is concluded that fetal histaminergic neurons survive transplantation to the adult hippocampal formation, and that this allograft procedure can supplement current strategies to investigate the function of histaminergic tuberomamillary neurons in the central nervous system.
开展了相关研究，以确定下丘脑后部结节乳头核中的胎儿组胺能神经元在颅内移植到成年宿主后是否能够存活。采用了两种移植方法。移植物要么被植入穹窿 - 海马伞损伤的延迟腔中，要么使用立体定位技术直接植入海马体。组织取自胚胎第16 - 17天的大鼠胎儿，并移植到斯普拉格 - 道利或费希尔344品系的成年大鼠体内。使用常规组织学和免疫组织化学方法评估移植物。所有移植到斯普拉格 - 道利大鼠的移植物都显示出排斥迹象，而在任何一只费希尔344大鼠中均未观察到排斥迹象。直接植入延迟的穹窿 - 海马伞腔中的移植物生长不如实质内移植物好，存活的神经元数量也不如实质内移植物多。将胚胎第17天的下丘脑后外侧块移植物直接植入费希尔344宿主的喙侧海马结构的CA1区域，获得了数量最多的存活组胺阳性神经元。在所有接受下丘脑移植物的费希尔344大鼠中都发现了具有神经突起生长的组胺免疫反应性细胞体。细胞体在整个细胞质中均匀地表现出组胺免疫反应性，并且具有与原位组胺能神经元相似的形态特征。轴突生长延伸至整个移植的下丘脑组织，有时在宿主海马组织中也可见。结论是胎儿组胺能神经元在移植到成年海马结构后能够存活，并且这种同种异体移植程序可以补充当前研究中枢神经系统中组胺能结节乳头神经元功能的策略。
