Curtis M, Bergman H, Price M L, Srivastava N, Granholm A C
Department of Basic Science, University of Colorado Health Sciences Center, Denver 80262, USA.
Hippocampus. 1995;5(6):584-94. doi: 10.1002/hipo.450050609.
The present study was undertaken to evaluate innervation and possible growth promotion by posterior hypothalamic tissue on different areas that are, or are not, interactive with this brain region during development. Posterolateral hypothalamus was dissected from embryonic day 17 rat fetuses, and inserted into the anterior chamber of the eye of adult rat hosts. Two weeks postgrafting, a second transplant consisting of either fetal hippocampal, cerebellar, or lung tissue was placed adjacent to the first graft. Growth of the intraocular double transplants was monitored weekly by measurements through the cornea. Fetal hippocampal tissue grew significantly larger when placed together with a hypothalamic graft, as compared to single hippocampal transplants. Cerebellar or lung tissue growth was not stimulated by a hypothalamic cograft. Pyramidal neuron cell counts demonstrated a significantly higher final number of these neurons in growth-stimulated hippocampal grafts, as compared to non-stimulated single hippocampal grafts. Immunohistochemistry with antibodies directed against histamine or histidine decarboxylase revealed that hippocampal transplants received the most dense histaminergic innervation. Cerebellar transplants contained occasional histaminergic neurites, and lung tissue never exhibited any histaminergic innervation from the adjacent hypothalamic graft. Taken together, these results demonstrate a growth-promoting effect of posterior hypothalamic tissue on developing hippocampal tissue, as well as target specificity of histaminergic innervation patterns.
本研究旨在评估下丘脑后部组织对不同区域的神经支配以及可能的生长促进作用，这些区域在发育过程中与该脑区存在或不存在相互作用。从胚胎第17天的大鼠胎儿中解剖出下丘脑后外侧，植入成年大鼠宿主的眼前房。移植两周后，将由胎儿海马、小脑或肺组织组成的第二次移植组织放置在第一次移植组织附近。通过角膜测量每周监测眼内双移植组织的生长情况。与单个海马移植相比，当与下丘脑移植一起放置时，胎儿海马组织生长得明显更大。下丘脑联合移植并未刺激小脑或肺组织的生长。锥体神经元细胞计数显示，与未受刺激的单个海马移植相比，生长受刺激的海马移植中这些神经元的最终数量明显更高。用针对组胺或组胺脱羧酶的抗体进行免疫组织化学分析表明，海马移植接受的组胺能神经支配最为密集。小脑移植偶尔含有组胺能神经突，而肺组织从未表现出来自相邻下丘脑移植的任何组胺能神经支配。综上所述，这些结果表明下丘脑后部组织对发育中的海马组织具有生长促进作用，以及组胺能神经支配模式的靶标特异性。
