Caruso M L, Pirrelli M, Armentano R
Servizio di Anatomia Patologica, Ospedale Gastroenterologico S. De Bellis, Castellana Grotte Bari, Italy.
Pathologica. 1994 Aug;86(4):376-83.
The most studied biological parameter of human tumours is the DNA nuclear content. In the present study we assessed the DNA content of 197 colorectal cancers from 180 patients using archival material (tissues routinely processed and paraffin-embedded) for image analysis. The metastatic lymph nodes were also included in the study in 54 Dukes "C" stage cases and 19/25 Dukes "D" stage cases. A total of 452 sections, 5 mu thick, were submitted to image analysis in static cytometry. DNA diploid pattern was found in 72/452 samples (15.9%) and the DNA-aneuploid pattern in 380/452 (84.1%). Two different areas for each tumour were examined but we found a concordance of diploid pattern in only 23 cases. DNA-aneuploidy, regarded as presence of at least one sample with an aneuploid peak in the same tumour, reached 88% in our study. In conclusion the image analysis of several samples obtained from different areas of the same tumour clearly revealed: a pronounced heterogeneity of ploidy patterns in colon cancer; an overall prevalence of DNA-aneuploidy in colon cancer; a prevalence of diploid pattern in tumours localized in right portion of the colon and in younger patients.
人类肿瘤中研究最多的生物学参数是DNA核含量。在本研究中，我们使用存档材料（常规处理并石蜡包埋的组织）对180例患者的197个结肠直肠癌进行了DNA含量评估，以进行图像分析。在54例杜克氏“C”期病例和19/25例杜克氏“D”期病例中，转移性淋巴结也纳入了研究。总共452个5微米厚的切片被提交用于静态细胞计数的图像分析。在452个样本中，72个（15.9%）发现DNA二倍体模式，380个（84.1%）发现DNA非整倍体模式。对每个肿瘤的两个不同区域进行了检查，但我们仅在23例中发现二倍体模式一致。在我们的研究中，DNA非整倍体（定义为同一肿瘤中至少有一个样本出现非整倍体峰）达到88%。总之，对同一肿瘤不同区域获取的多个样本进行图像分析清楚地显示：结肠癌中倍体模式存在明显异质性；结肠癌中DNA非整倍体总体上占优势；位于结肠右侧的肿瘤以及年轻患者的肿瘤中二倍体模式占优势。
