Martin F, Eriani G, Reinbolt J, Dirheimer G, Gangloff J
Unité Propre de Recherche 9002 du Centre National de la Recherche Scientifique, Strasbourg, France.
Nucleic Acids Res. 1995 Mar 11;23(5):779-84. doi: 10.1093/nar/23.5.779.
Suppressor tRNAs are useful tools for determining identity elements which define recognition of tRNAs in vivo by their cognate aminoacyl-tRNA synthetases. This study was aimed at the isolation of active amber tRNA(Asn). Nineteen mutated tRNA(Asn)CUA having amber suppressor activity were selected by an in vivo genetic screen, and all exclusively inserted glutamine. From analysis of the different mutations it is concluded that glutamine accepting activity was obtained upon reducing the interaction strength between the first base pair of the tRNA(Asn)CUA by direct or indirect effects. Failure to isolate tRNA(Asn)CUA suppressors charged with asparagine as well as other evolutionary related amino acids is discussed.
抑制性tRNA是用于确定识别元件的有用工具,这些识别元件在体内定义了其同源氨酰-tRNA合成酶对tRNA的识别。本研究旨在分离活性琥珀色tRNA(Asn)。通过体内遗传筛选选择了19种具有琥珀色抑制活性的突变tRNA(Asn)CUA,并且它们都只插入谷氨酰胺。通过对不同突变的分析得出结论,通过直接或间接作用降低tRNA(Asn)CUA第一个碱基对之间的相互作用强度后,获得了谷氨酰胺接受活性。讨论了未能分离出携带天冬酰胺以及其他进化相关氨基酸的tRNA(Asn)CUA抑制子的情况。