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两条螺旋加一个连接体：真核核糖核酸酶P的一个小型模型底物。

Two helices plus a linker: a small model substrate for eukaryotic RNase P.

作者信息

Carrara G, Calandra P, Fruscoloni P, Tocchini-Valentini G P

机构信息

Istituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche, Rome, Italy.

出版信息

Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2627-31. doi: 10.1073/pnas.92.7.2627.

DOI:10.1073/pnas.92.7.2627
PMID:7708695
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC42271/
Abstract

Using precursor tRNA molecules to study RNA-protein interactions, we have identified an RNA motif recognized by eukaryotic RNase P (EC 3.1.26.5). Analysis of circularly permuted precursors indicates that interruptions in the sugar-phosphate backbone are not tolerated in the acceptor stem, in the T stem-loop, or between residues A-9 and G-10. Prokaryotic RNase P will function with a minihelix consisting of the acceptor stem connected directly to the T stem-loop. Eukaryotic RNase P cannot use such a minimal substrate unless a linker sequence is added in the gap where the D stem and anticodon stem-loop were deleted.

摘要

利用前体tRNA分子研究RNA-蛋白质相互作用，我们鉴定出了一种真核核糖核酸酶P（EC 3.1.26.5）识别的RNA基序。对环形排列的前体的分析表明，在受体茎、T茎环或A-9和G-10残基之间，磷酸核糖主链的中断是不被允许的。原核核糖核酸酶P可以与一个由直接连接到T茎环的受体茎组成的小螺旋起作用。真核核糖核酸酶P不能使用这样的最小底物，除非在缺失D茎和反密码子茎环的间隙中添加一个接头序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8008/42271/74d11d3ec9ea/pnas01485-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8008/42271/6681a21a6718/pnas01485-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8008/42271/74d11d3ec9ea/pnas01485-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8008/42271/6681a21a6718/pnas01485-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8008/42271/74d11d3ec9ea/pnas01485-0222-a.jpg

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