Sachs R K, van den Engh G, Trask B, Yokota H, Hearst J E
Department of Mathematics, University of California, Berkeley 94720, USA.
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2710-4. doi: 10.1073/pnas.92.7.2710.
Fluorescence in situ hybridization data on distances between defined genomic sequences are used to construct a quantitative model for the overall geometric structure of a human chromosome. We suggest that the large-scale geometry during the G0/G1 part of the cell cycle may consist of flexible chromatin loops, averaging approximately 3 million bp, with a random-walk backbone. A fully explicit, three-parametric polymer model of this random-walk/giant-loop structure can account well for the data. More general models consistent with the data are briefly discussed.
关于特定基因组序列间距离的荧光原位杂交数据被用于构建人类染色体整体几何结构的定量模型。我们认为，细胞周期G0/G1期的大规模几何结构可能由平均约300万碱基对的柔性染色质环组成，其主干为随机游走形式。这种随机游走/巨环结构的一个完全显式的三参数聚合物模型能够很好地解释这些数据。还简要讨论了与数据相符的更通用模型。
