枯草芽孢杆菌中Spo0A转录因子的激活需要三羧酸循环功能。
Krebs cycle function is required for activation of the Spo0A transcription factor in Bacillus subtilis.
作者信息
Ireton K, Jin S, Grossman A D, Sonenshein A L
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
出版信息
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2845-9. doi: 10.1073/pnas.92.7.2845.
Abstract
Expression of genes early during sporulation in Bacillus subtilis requires the activity of the transcription factor encoded by spo0A. The active, phosphorylated form of Spo0A is produced through the action of a multicomponent pathway, the phosphorelay. A mutant defective in the first three enzymes of the Krebs citric acid cycle was unable to express early sporulation genes, apparently because of a failure to activate the phosphorelay. Cells that produce an altered Spo0A protein that can be phosphorylated by an alternative pathway were not dependent on Krebs cycle function for early sporulation gene expression. These findings suggest that Krebs cycle enzymes transmit a signal to activate the phosphorelay and that B. subtilis monitors its metabolic potential before committing itself to spore formation.
摘要
枯草芽孢杆菌孢子形成早期基因的表达需要由spo0A编码的转录因子的活性。Spo0A的活性磷酸化形式是通过多组分途径——磷酸转移中继系统产生的。三羧酸循环前三种酶有缺陷的突变体无法表达孢子形成早期基因，这显然是因为未能激活磷酸转移中继系统。产生一种可通过替代途径磷酸化的改变型Spo0A蛋白的细胞，在孢子形成早期基因表达方面不依赖于三羧酸循环功能。这些发现表明，三羧酸循环酶传递激活磷酸转移中继系统的信号，并且枯草芽孢杆菌在决定形成孢子之前会监测其代谢潜力。
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