13-氢过氧十八碳二烯酸对兔胃窦黏膜中15-羟基前列腺素脱氢酶活性的抑制作用。

Inhibition of 15-hydroxy prostaglandin dehydrogenase activity in rabbit gastric antral mucosa by 13-hydroperoxyoctadecadienoic acid.

作者信息

Sakuma S, Fujimoto Y, Miyata Y, Yamane K, Nishida H, Fujita T

机构信息

Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 1994 Dec;51(6):425-30. doi: 10.1016/0952-3278(94)90060-4.

DOI:10.1016/0952-3278(94)90060-4

PMID:7708809

Abstract

The effect of 13-hydroperoxyoctadecadienoic acid (13-HPODE), a hydroperoxy adduct of linoleic acid (LA), on the activities of prostaglandin (PG) synthesizing and catabolizing enzymes in rabbit gastric antral mucosa was examined. 13-HPODE had no effect on the synthesis of PGE2, PGF2 alpha and PGD2 from exogenous arachidonic acid in the microsomal fraction of the gastric mucosa at concentrations ranging from 5-20 microM. On the other hand, at 1-10 microM, it inhibited the activity of 15-hydroxy PG dehydrogenase (PGDH), which catalyzes the initial step of catabolism of PGs, in a dose-dependent manner. The concentration required for 50% inhibition was approximately 1 microM. Experiments utilizing LA, 13-hydroperoxyoctadecadienoic acid and Fe2+ indicated the requirement of the hydroperoxy moiety for the inhibitory effect of 13-HPODE on the PGDH activity. These results suggest that 13-HPODE has the potential to increase the levels of biologically active PGs in gastric mucosa by preventing their inactivation and may have functional effects within the stomach.

摘要

研究了亚油酸（LA）的氢过氧化物加合物13 - 氢过氧十八碳二烯酸（13 - HPODE）对兔胃窦黏膜中前列腺素（PG）合成酶和分解代谢酶活性的影响。在5 - 20微摩尔浓度范围内，13 - HPODE对胃黏膜微粒体部分中外源性花生四烯酸合成PGE2、PGF2α和PGD2没有影响。另一方面，在1 - 10微摩尔时，它以剂量依赖的方式抑制15 - 羟基PG脱氢酶（PGDH）的活性，该酶催化PG分解代谢的起始步骤。50%抑制所需的浓度约为1微摩尔。利用LA、13 - 氢过氧十八碳二烯酸和Fe2+进行的实验表明，13 - HPODE对PGDH活性的抑制作用需要氢过氧基部分。这些结果表明，13 - HPODE有可能通过防止生物活性PG失活来增加胃黏膜中其水平，并且可能在胃内具有功能作用。