Hexeberg S, Willumsen N, Berge R K
Department of Anatomy and Cell Biology, University of Bergen, Norway.
Scand J Clin Lab Invest. 1994 Dec;54(8):665-71. doi: 10.3109/00365519409087548.
The aim of the study was to explore whether treatment with highly purified docosahexaenoic acid (DHA) over a short period affects the amount of lipid droplets in myocardial cells of rats, and whether heart peroxisomal enzyme activity is changed. Fifteen rats were fed a standard diet for 10 days and 15 rats were fed a cholesterol diet (2% of cholesterol) for 10 days. In each experiment six rats served as control, and three rats in each treatment group were given one of the following treatments by gastric intubation: DHA at 500, 1000, or 1500 mg day-1 kg-1 body weight. The fractional volume of lipid droplets in myocardial cells was calculated by morphometric methods. The heart triglycerides and the volume fraction of lipid droplets in the myocardium were greater in the standard diet rats treated with DHA compared with controls. There was no such increase caused by DHA treatment in the cholesterol diet rats. The heart fatty acyl-CoA oxidase tended to increase with DHA treatment in both standard and cholesterol diet rats, but this was significantly increased only after treatment with DHA 1500 mg day-1 kg-1 in the cholesterol diet rats. We conclude that treatment with highly purified DHA for 10 days results in cardiac lipidosis, assessed both by biochemical and morphological methods in standard diet rats, whereas DHA treatment has no additive effect on lipid accumulation in cholesterol fed rats.
本研究的目的是探讨短期内用高度纯化的二十二碳六烯酸（DHA）进行治疗是否会影响大鼠心肌细胞中脂滴的数量，以及心脏过氧化物酶体酶活性是否会发生变化。15只大鼠喂食标准饮食10天，15只大鼠喂食含2%胆固醇的饮食10天。在每个实验中，6只大鼠作为对照，每个治疗组中的3只大鼠通过胃插管给予以下治疗之一：剂量为500、1000或1500毫克/天·千克体重的DHA。通过形态计量学方法计算心肌细胞中脂滴的体积分数。与对照组相比，用DHA治疗的标准饮食大鼠心肌中的甘油三酯和脂滴体积分数更高。胆固醇饮食大鼠经DHA治疗后未出现这种增加。标准饮食和胆固醇饮食大鼠经DHA治疗后，心脏脂肪酸酰基辅酶A氧化酶均有升高趋势，但仅在胆固醇饮食大鼠中给予1500毫克/天·千克体重的DHA治疗后，该酶活性才显著升高。我们得出结论，用高度纯化的DHA治疗10天会导致标准饮食大鼠出现心脏脂质沉积，这可通过生化和形态学方法进行评估，而DHA治疗对喂食胆固醇的大鼠的脂质积累没有附加作用。
