Hexeberg S, Willumsen N, Berge R K
Department of Anatomy and Cell Biology, University of Bergen, Norway.
Biochim Biophys Acta. 1995 Jun 6;1256(3):341-5. doi: 10.1016/0005-2760(95)00043-c.
Rats were given eicosapentaenoic acid (EPA) or palmitic acid (PALM) up to 15 days, and control animals were given carboxymethylcellulose. All suspensions which were given by gastric intubation contained tocopherol. Heart triacylglycerols, heart cholesterol and heart phospholipids significantly increased after one day of EPA treatment, but they were normalized within 15 days. Both after 2 and 10 days of treatment with palmitic acid the heart triacylglycerols were significantly greater than control. The heart cholesterol and heart phospholipids were significantly greater than control after 10 days of treatment with palmitic acid. Total carnitine palmitoyltransferase (CPT) activity in heart was significantly greater in rats treated with EPA for 15 days compared to control, but treatment with palmitic acid had no effect. The fatty acyl-CoA oxidase activity was greater in rats treated with EPA for 15 days and palmitic acid for 10 days compared to control. The fractional volume of lipid droplets in myocardial cells was calculated from electronmicrographs and was 0.112 +/- 0.016% after 1 day of EPA treatment compared to 0.035 +/- 0.016% in the control group. After 5 and 15 days the fractional volume was the same as control. The fractional volume of lipid droplets in rats treated with palmitic acid for 10 days was 0.120 +/- 0.023%. Treatment with EPA caused an immediate accumulation of lipids and lipid droplets in the rat heart which after few days normalized in parallel with an increased activity of total CPT in the heart.
给大鼠喂食二十碳五烯酸（EPA）或棕榈酸（PALM）长达15天，对照组动物喂食羧甲基纤维素。所有经胃插管给予的混悬液中均含有生育酚。EPA处理1天后，心脏三酰甘油、心脏胆固醇和心脏磷脂显著增加，但在15天内恢复正常。棕榈酸处理2天和10天后，心脏三酰甘油均显著高于对照组。棕榈酸处理10天后，心脏胆固醇和心脏磷脂显著高于对照组。与对照组相比，经EPA处理15天的大鼠心脏中总肉碱棕榈酰转移酶（CPT）活性显著更高，但棕榈酸处理则无此效果。与对照组相比，经EPA处理15天和棕榈酸处理10天的大鼠脂肪酰基辅酶A氧化酶活性更高。根据电子显微镜照片计算心肌细胞中脂滴的分数体积，EPA处理1天后为0.112±0.016%，而对照组为0.035±0.016%。5天和15天后，分数体积与对照组相同。棕榈酸处理10天的大鼠脂滴分数体积为0.120±0.023%。EPA处理导致大鼠心脏中脂质和脂滴立即积累，几天后恢复正常，同时心脏中总CPT活性增加。
