dl-前胡素A对豚鼠心室肌细胞钙电流的影响。
Effect of dl-praeruptorin A on calcium current in ventricular cells of guinea pig.
作者信息
Li J M, Chang T H, Sun X D, Hao L Y, Wang Y P, Yu Y F, Zhang K Y
机构信息
Department of Pharmacology, China Medical University, Shenyang.
出版信息
Zhongguo Yao Li Xue Bao. 1994 Nov;15(6):525-7.
With patch clamp technic (whole cell recording), the effect of dl-praeruptorin A (Pra), an ingredient of Peucedanum praeruptorum Dunn on calcium current (ICa) in the single ventricular cells of guinea pig was studied. Results showed that under Cs/Cs condition, when the holding potential was -40 mV and in the presence of Pra (1, 10, 100 mumol.L-1), ICa was decreased dose-dependently from 2.02 +/- 0.24, 2.00 +/- 0.12, 2.12 +/- 0.33 nA (control) to 1.60 +/- 0.24, 1.32 +/- 0.08, 1.16 +/- 0.43 nA, respectively, and their inhibitory rates were 21%, 33.5%, 45%, respectively. The current-voltage relation curve showed that the reversal potential of ICa was +60 mV; the potential producing peak value of ICa was about 0 mV. The results indicated that Pra had a Ca2+ channel blocking effect.
采用膜片钳技术（全细胞记录模式），研究了白花前胡的成分dl-前胡素A（Pra）对豚鼠单个心室肌细胞钙电流（ICa）的影响。结果显示，在Cs/Cs条件下，当钳制电压为-40 mV且存在Pra（1、10、100 μmol·L-1）时，ICa分别从对照时的2.02±0.24、2.00±0.12、2.12±0.33 nA呈剂量依赖性降低至1.60±0.24、1.32±0.08、1.16±0.43 nA，其抑制率分别为21%、33.5%、45%。电流-电压关系曲线表明，ICa的反转电位为+60 mV；产生ICa峰值的电位约为0 mV。结果提示，Pra具有钙通道阻滞作用。
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