School of Pharmaceutical Science, Shanxi Medical University, No 56, Xinjian Nan Road, Taiyuan 030001, Shanxi, China.
Molecules. 2010 Nov 9;15(11):8060-71. doi: 10.3390/molecules15118060.
In this study, the effects of Peucedanum praeruptorum DUNN methanolic extract (PPME) and its major constituents on SGC7901 human gastric cancer cells were evaluated. Two pyranocoumarins, namely, (±) praeruptorin A (PA) and (±) praeruptorin B (PB), were isolated from PPME. A high performance liquid chromatographic (HPLC) method was developed to determine the contents of PA and PB in PPME. The anti-proliferative and cytotoxic actions of PPME were observed using the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and release of lactate dehydrogenase (LDH) assays. At 300 μg/mL, PPME inhibited cell growth by 51.2% (P < 0.01), probably linked to the high concentration of PA and PB. Both PA and PB exhibited antiproliferative and cytotoxic activities on the SGC7901 cells. Furthermore, the active principle compound, PA, also enhanced the actions of doxorubincin (DOX) on SGC7901 cells. Cell growth decreased higher with the combined treatment of PA and DOX than that with the chemotherapy agent applied alone, suggesting that PA could reduce the dose of DOX for the desired effects.
在这项研究中，评估了白芷甲醇提取物（PPME）及其主要成分对 SGC7901 人胃癌细胞的影响。从 PPME 中分离出两种吡喃香豆素，即（±）前胡素 A（PA）和（±）前胡素 B（PB）。建立了一种高效液相色谱（HPLC）方法来测定 PPME 中 PA 和 PB 的含量。使用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）和乳酸脱氢酶（LDH）释放测定法观察 PPME 的抗增殖和细胞毒性作用。在 300μg/mL 时，PPME 抑制细胞生长 51.2%（P<0.01），可能与 PA 和 PB 的高浓度有关。PA 和 PB 均对 SGC7901 细胞表现出增殖抑制和细胞毒性作用。此外，活性化合物 PA 还增强了阿霉素（DOX）对 SGC7901 细胞的作用。与单独使用化疗药物相比，PA 和 DOX 联合治疗时细胞生长下降更高，这表明 PA 可以降低 DOX 的剂量以达到所需的效果。
