Gasser R B, Parada L, Acuna A, Burges C, Laurenson M K, Gulland F M, Reichel M P, Paolillo E
Department of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia.
Acta Trop. 1994 Dec;58(3-4):179-85. doi: 10.1016/0001-706x(94)90012-4.
An ELISA was used to screen a dog population in Uruguay (Sarandi Del Yi, Durazno District) for the prevalence of specific serum antibodies (IgG, IgA and IgE) to Echinococcus granulosus. The sensitivity (61%) and specificity (97%) of the ELISA were determined using well-defined serum groups. A total of 408 dogs from Sarandi del Yi and environs were screened serologically, and 29.7% (8.6-13.8% for each antibody class) of dogs had positive levels of antibody to E. granulosus. This antibody prevalence (exposure) was significantly higher than the percentage of dogs found to be positive for E. granulosus worms by arecoline purgation (7.6%). This level of exposure to E. granulosus determined by ELISA is considered unacceptable from a public health perspective. Measures will now focus on obtaining data on the true prevalence of current infection in this dog population and on determining the transmission patterns of the disease in this endemic region.
采用酶联免疫吸附测定法（ELISA）对乌拉圭（杜拉佐区萨兰迪德尔伊）的犬类群体进行筛查，以确定细粒棘球绦虫特异性血清抗体（IgG、IgA和IgE）的流行情况。使用明确界定的血清组确定ELISA的敏感性（61%）和特异性（97%）。对来自萨兰迪德尔伊及其周边地区的408只犬进行了血清学筛查，29.7%的犬（每种抗体类别为8.6 - 13.8%）细粒棘球绦虫抗体水平呈阳性。这种抗体流行率（暴露率）显著高于通过槟榔碱驱虫法发现的细粒棘球绦虫成虫阳性犬的百分比（7.6%）。从公共卫生角度来看，ELISA测定的这种细粒棘球绦虫暴露水平被认为是不可接受的。现在将重点采取措施获取该犬类群体当前感染真实流行情况的数据，并确定该流行地区疾病的传播模式。
