激光能量与染料荧光在体外透过血液的传输情况。

Laser energy and dye fluorescence transmission through blood in vitro.

作者信息

Cohen S M, Shen J H, Smiddy W E

机构信息

Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida, USA.

出版信息

Am J Ophthalmol. 1995 Apr;119(4):452-7. doi: 10.1016/s0002-9394(14)71231-0.

DOI:10.1016/s0002-9394(14)71231-0

PMID:7709969

Abstract

PURPOSE

Because of the potential usefulness of evaluating and treating choroidal neovascularization obscured by blood, we designed this study to quantify the transmission of dye fluorescence and laser energy through blood.

METHODS

Blood preparations anticoagulated with ethylenediaminetetraacetic acid with hematocrits of 0% (plasma), 46%, and 99% were placed in open cuvettes with path lengths of 100, 200, or 500 microns and were exposed for one minute to either 100% oxygen or 100% carbon dioxide. Each cuvette was then sealed. Photographs of the cuvettes of blood in front of a flask of fluorescein or indocyanine green solution were decoded and used to calculate the percent transmission of fluorescence through blood. Cuvettes of blood were also placed in the path of argon, krypton, and diode lasers for energy transmission measurements.

RESULTS

Plasma transmission of fluorescein and indocyanine green fluorescence and argon, krypton, and diode laser energy was 89% to 100% for all samples tested. Transmission of fluorescein fluorescence and argon laser energy through 99% hematocrit samples were both less than 5%. Transmission of indocyanine green fluorescence through 100-, 200-, and 500-microns-thick cuvettes filled with 99% hematocrit blood was 57%, 34%, and 4%. Transmission of krypton laser energy was 50%, 25%, and 6%; and transmission of diode laser energy was 60%, 35%, and 12% through 99% hematocrit blood. Intermediate transmission values were obtained for 46% hematocrit samples.

CONCLUSIONS

Krypton and, to a slightly greater degree, diode laser energy penetrate a thin film of blood. Indocyanine green fluorescence also penetrates a thin film of blood. If a layer of blood appears thinner than 500 microns, then indocyanine green angiography may be useful in imaging underlying pathologic features. If a lesion can be imaged with indocyanine green, then it can probably be treated with a krypton or diode laser.

摘要

目的

鉴于评估和治疗被血液遮盖的脉络膜新生血管具有潜在的实用价值，我们设计了本研究来量化染料荧光和激光能量透过血液的情况。

方法

将用乙二胺四乙酸抗凝、血细胞比容分别为0%（血浆）、46%和99%的血液样本置于光程为100、200或500微米的开放式比色皿中，分别暴露于100%氧气或100%二氧化碳环境中一分钟。然后将每个比色皿密封。对置于装有荧光素或吲哚菁绿溶液的烧瓶前的血液比色皿进行拍照并解码，用于计算荧光透过血液的百分比。血液比色皿还被置于氩、氪和二极管激光的光路中进行能量传输测量。

结果

对于所有测试样本，荧光素和吲哚菁绿荧光以及氩、氪和二极管激光能量在血浆中的透过率为89%至100%。荧光素荧光和氩激光能量透过血细胞比容为99%的样本的透过率均低于5%。吲哚菁绿荧光透过装有血细胞比容为99%血液的100微米、200微米和500微米厚比色皿的透过率分别为57%、34%和4%。氪激光能量的透过率分别为50%、25%和6%；二极管激光能量透过血细胞比容为99%血液的透过率分别为60%、35%和12%。血细胞比容为46%的样本获得了中间透过率值。