Matson R S, Rampal J, Pentoney S L, Anderson P D, Coassin P
Advanced Technology Center, Beckman Instruments, Incorporated, Fullerton, California 92634.
Anal Biochem. 1995 Jan 1;224(1):110-6. doi: 10.1006/abio.1995.1014.
High-density oligonucleotide arrays have been constructed on clear, aminated polypropylene film using conventional phosphoramidite-based synthesis chemistries. A semiautomated 64-channel fluidic chemical delivery system is used to prepare the 64 x 64 array containing 4096 oligonucleotide elements. The completed array has been successfully used in the hybridization and fluorescence (Streptavidin-FITC) detection of a biotinylated 18-mer oligonucleotide target complementary to the F508 codon region of the cystic fibrosis transmembrane conductance regulator gene. A hybridization simulation model based upon Nearest Neighbor approximations is used to predict the outcome of the array hybridization experiment.
高密度寡核苷酸阵列已使用基于亚磷酰胺的传统合成化学方法在透明的、胺化聚丙烯薄膜上构建。一个半自动的64通道流体化学输送系统用于制备包含4096个寡核苷酸元件的64×64阵列。完整的阵列已成功用于与囊性纤维化跨膜传导调节基因F508密码子区域互补的生物素化18聚体寡核苷酸靶标的杂交和荧光（链霉亲和素 - 异硫氰酸荧光素）检测。基于最近邻近似的杂交模拟模型用于预测阵列杂交实验的结果。
