Matson R S, Rampal J B, Coassin P J
Advanced Development Unit, Beckman Instruments, Fullerton, California 92634.
Anal Biochem. 1994 Mar;217(2):306-10. doi: 10.1006/abio.1994.1123.
We have modified polypropylene to serve as a new solid-phase support for oligonucleotide synthesis. The plastic is first surface aminated by exposure to an ammonia plasma generated by radiofrequency plasma discharge. The aminated polypropylene has been found to be useful as a support for the in situ synthesis of oligonucleotides from monomers. Furthermore, oligonucleotides synthesized on the surface of the plastic remain attached following deprotection and can be used directly for hybridization.
我们已对聚丙烯进行改性，使其成为用于寡核苷酸合成的新型固相载体。首先通过暴露于射频等离子体放电产生的氨等离子体对该塑料进行表面胺化。已发现胺化聚丙烯可作为从单体原位合成寡核苷酸的载体。此外，在塑料表面合成的寡核苷酸在脱保护后仍保持附着状态，可直接用于杂交。
