Zhang J, Tsuboyama N, Kodama H
Department of Chemistry, Kochi Medical School, Japan.
Anal Biochem. 1995 Jan 1;224(1):17-20. doi: 10.1006/abio.1995.1002.
A method for simultaneous determination of cystathionine and NAc-cystathionine in biological samples has been developed by using column liquid chromatography-mass spectrometry. The concentrations of cystathionine and NAc-cystathionine in urine, several tissues, and serum of propargylglycine-treated rats with experimental cystathioninuria were determined by scanning the [M + H]+ ions of each compound. The recoveries of authentic compounds were 95.69 +/- 4.44% (n = 10) for cystathionine and 91.59 +/- 5.26% (n = 10) for NAc-cystathionine after ion-exchange resin treatment. The contents of cystathionine and NAc-cystathionine in the urine of a patient with cystathioninuria were also measured. The results coincided well with those obtained using an amino acid analyzer.
已开发出一种利用柱液相色谱-质谱法同时测定生物样品中胱硫醚和N-乙酰胱硫醚的方法。通过扫描每种化合物的[M + H]+离子，测定了患有实验性胱硫醚尿症的炔丙基甘氨酸处理大鼠的尿液、几种组织和血清中胱硫醚和N-乙酰胱硫醚的浓度。经离子交换树脂处理后，胱硫醚的真实化合物回收率为95.69±4.44%（n = 10），N-乙酰胱硫醚的回收率为91.59±5.26%（n = 10）。还测定了一名胱硫醚尿症患者尿液中胱硫醚和N-乙酰胱硫醚的含量。结果与使用氨基酸分析仪获得的结果非常吻合。
