Dybczyński R, Danko B
Department of Analytical Chemistry, Institute of Nuclear Chemistry & Technology, Warszawa, Poland.
Biol Trace Elem Res. 1994 Fall;43-45:615-25. doi: 10.1007/BF02917364.
A newly devised, very accurate ("definitive") method for the determination of trace amounts of cobalt in biological materials was validated by the analysis of several certified reference materials. The method is based on a combination of neutron activation and selective and quantitative postirradiation isolation of radiocobalt from practically all other radionuclides by ion-exchange and extraction chromatography followed by gamma-ray spectrometric measurement. The significance of criteria that should be fulfilled in order to accept a given result as obtained by the "definitive method" is emphasized. In view of the demonstrated very good accuracy of the method, it is suggested that our values for cobalt content in those reference materials in which it was originally not certified (SRM 1570 spinach, SRM 1571 orchard leaves, SRM 1577 bovine liver, and Czechoslovak bovine liver 12-02-01) might be used as provisional certified values.
一种新设计的、非常精确（“权威”）的测定生物材料中痕量钴的方法，通过对几种有证标准物质的分析进行了验证。该方法基于中子活化，以及通过离子交换和萃取色谱法从几乎所有其他放射性核素中选择性定量地分离出辐照后的放射性钴，随后进行伽马射线光谱测量。强调了为将“权威方法”获得的给定结果视为可接受而应满足的标准的重要性。鉴于该方法已证明具有非常好的准确性，建议将我们测定的那些最初未认证钴含量的标准物质（SRM 1570菠菜、SRM 1571果园树叶、SRM 1577牛肝和捷克斯洛伐克牛肝12 - 02 - 01）中的钴含量值用作临时认证值。
