Hussain I, Plevris J N, Hayes P C, Bouchier I A
Department of Medicine, Royal Infirmary, Edinburgh, UK.
Eur J Gastroenterol Hepatol. 1995 Feb;7(2):141-4.
To determine whether acid secretion by gall bladder mucosa is influenced by alcohol and whether hydrogen ion output is increased when the gall bladder is perfused with moderate concentrations of alcohol.
Fifty bovine gall bladders were studied. Twenty-five served as controls and 25 were perfused (in groups of five) with varying concentrations of alcohol (w/v): 0.05, 0.1, 0.2, 0.5 and 1.0%. Acid output was measured over 1 h and the results expressed as the mean.
There was no significant difference between acid production by control gall bladders and those perfused with 0.05% alcohol (24.30 +/- 8.7 versus 18.95 x 8.41 nmol/h), but the acid output was significantly increased when the mucosa was exposed to 0.1 and 0.2% alcohol concentrations (50.28 +/- 10.2 and 29.34 +/- 9.1 nmol/h; P < 0.001 and P < 0.0004, respectively). Perfusion with alcohol concentrations of 0.5 and 1.0%, significantly inhibited mucosal acid output (2.60 +/- 2.0 and -1.70 +/- 1.2 nmol/h; P < 0.0001 and P < 0.0001, respectively).
This study shows that perfusion of bovine gall bladder mucosa in vitro with moderate concentrations of alcohol stimulates acid output. This observation is important for assessing the link between alcohol and the risk of gallstone formation.
确定酒精是否会影响胆囊黏膜的酸分泌，以及当胆囊用中等浓度酒精灌注时氢离子输出量是否会增加。
对50个牛胆囊进行研究。25个作为对照，25个（每组5个）用不同浓度（重量/体积）的酒精灌注：0.05%、0.1%、0.2%、0.5%和1.0%。在1小时内测量酸输出量，并将结果表示为平均值。
对照胆囊与用0.05%酒精灌注的胆囊之间的酸产生量无显著差异（24.30±8.7对18.95×8.41纳摩尔/小时），但当黏膜暴露于0.1%和0.2%酒精浓度时，酸输出量显著增加（50.28±10.2和29.34±9.1纳摩尔/小时；分别为P<0.001和P<0.0004）。用0.5%和1.0%酒精浓度灌注显著抑制黏膜酸输出（2.60±2.0和-1.70±1.2纳摩尔/小时；分别为P<0.0001和P<0.0001）。
本研究表明，在体外对牛胆囊黏膜用中等浓度酒精灌注会刺激酸输出。这一观察结果对于评估酒精与胆结石形成风险之间的联系很重要。
