Schröder D, Hehmke B, Klöting I, Schmidt S
Institute of Diabetes Gerhardt Katsch Karlsburg, Ernst-Moritz-Arndt-University, Greifswald, Germany.
Diabetes Res. 1993;23(3):105-13.
Diabetes-prone BB/OK rats were checked in a follow-up study between 40 and 200 days of age for the appearance of humoral-mediated cytotoxicity to rat pancreatic exocrine cells in serum by 51Cr-release assay. BB/OK rats maintaining normoglycaemia were characterized by a pronounced decrease of anti-exocrine cell cytotoxicity in serum at 75 and 105 days of age compared to the initial value at day 40. In contrast, in animals developing diabetes cytotoxicity was found more frequently and the level of cytotoxicity did not likewise decrease during the period up to diabetes onset. With respect to the prediction of diabetes in individual BB/OK rats the retrospective analysis revealed that 81.0% of those animals displaying cytotoxicity at 75 days of age subsequently developed diabetes. If sera from BB/OK rats were identified as non-cytotoxic at day 75 and also at 105 days of age normoglycaemia persisted in 83.3% of these animals. Thus, screening of young diabetes-prone BB/OK rats for the presence of humoral-mediated cytotoxicity to pancreatic exocrine cells in serum seems to facilitate the identification of potential diabetic or long-term normoglycaemic animals in the BB/OK rat population.
在一项随访研究中，对易患糖尿病的BB/OK大鼠在40至200日龄期间进行检查，通过51Cr释放试验检测血清中对大鼠胰腺外分泌细胞的体液介导细胞毒性的出现情况。维持血糖正常的BB/OK大鼠的特征是，与40日龄时的初始值相比，75日龄和105日龄时血清中抗外分泌细胞的细胞毒性明显降低。相比之下，在发生糖尿病的动物中，细胞毒性更频繁地被发现，并且在糖尿病发病前的这段时间内，细胞毒性水平并未同样降低。关于个体BB/OK大鼠糖尿病的预测，回顾性分析显示，75日龄时表现出细胞毒性的动物中，81.0%随后发生了糖尿病。如果BB/OK大鼠的血清在75日龄和105日龄时被鉴定为无细胞毒性，这些动物中有83.3%的血糖仍保持正常。因此，对易患糖尿病的幼龄BB/OK大鼠血清中对胰腺外分泌细胞的体液介导细胞毒性进行筛查，似乎有助于在BB/OK大鼠群体中识别潜在的糖尿病动物或长期血糖正常的动物。
