Li J, Liu Y L, Sha J Y
Changhai Hospital, Second Military Medical University, Shanghai.
Zhonghua Fu Chan Ke Za Zhi. 1994 Nov;29(11):670-2, 700.
Two ovarian cancer cell lines, 3AO and NIH: OVCAR-3, were used in this study to assess the synergistic effect of TNF and IFN gamma in combination. The result showed that these two ovarian cancer cell lines were all resistant to TNF and IFN gamma individually, but sensitive to the combined administration of TNF and IFN gamma. Concentration of 1 x 10(4) U/L of TNF and IFN gamma significantly inhibited 3AO and NIH: OVCAR-3 ovarian cancer cells (P < 0.05). Administration of 1 x 10(6) U/L TNF and IFN gamma could inhibit the growth of 3AO cells by 55% and NIH: OVCAR-3 cells by 75% (P < 0.01). It suggests that in some ovarian cancer cells which resistant to TNF could be changed to TNF sensitive cells by IFN gamma. anticancer treatment higher antitumor activity could be achieved by combined administration of TNF and IFN gamma.
本研究使用了两种卵巢癌细胞系3AO和NIH:OVCAR-3，以评估肿瘤坏死因子（TNF）和γ干扰素（IFNγ）联合使用的协同效应。结果显示，这两种卵巢癌细胞系对TNF和IFNγ单独使用均具有抗性，但对TNF和IFNγ联合给药敏感。浓度为1×10⁴U/L的TNF和IFNγ显著抑制了3AO和NIH:OVCAR-3卵巢癌细胞（P<0.05）。给予1×10⁶U/L的TNF和IFNγ可使3AO细胞的生长抑制55%，NIH:OVCAR-3细胞的生长抑制75%（P<0.01）。这表明在一些对TNF具有抗性的卵巢癌细胞中，可通过IFNγ使其转变为对TNF敏感的细胞。通过TNF和IFNγ联合给药可实现更高的抗癌治疗抗肿瘤活性。
