Taguchi T, Kimoto Y, Tanji Y, Abe S, Nakano K, Sohmura Y
Department of Surgery, Osaka University, Japan.
J Biol Response Mod. 1987 Dec;6(6):599-609.
The present study was undertaken to examine the effect of recombinant human interferon-gamma (rHu-IFN-gamma) on the in vitro antitumor activity of recombinant human tumor necrosis factor (rHu-TNF) against rHu-TNF-sensitive and resistant tumor cells. rHu-IFN-gamma augmented both cytostatic and cytocidal activity of rHu-TNF. When 14 tumor cells were tested, augmentation by rHu-IFN-gamma was observed in most of rHu-TNF-sensitive tumor cells and in few cases in rHu-TNF-resistant tumor cells. Among rHu-TNF-sensitive tumor cells, there was no relationship between the degree of augmentation and the susceptibility to rHu-TNF. Augmentation was marked when tumor cells were treated with rHu-IFN-gamma either before the exposure to rHu-TNF or during the early period of the exposure to rHu-TNF. On the other hand, augmentation was not observed when tumor cells were treated with rHu-IFN-gamma during a late period of the exposure to rHu-TNF. From these results, a combined treatment with both agents can be expected to provide an approach to get better results in clinical trials.
本研究旨在检测重组人干扰素-γ（rHu-IFN-γ）对重组人肿瘤坏死因子（rHu-TNF）体外抗rHu-TNF敏感和耐药肿瘤细胞活性的影响。rHu-IFN-γ增强了rHu-TNF的细胞生长抑制和细胞杀伤活性。在检测的14种肿瘤细胞中，大多数rHu-TNF敏感肿瘤细胞中观察到rHu-IFN-γ的增强作用，少数rHu-TNF耐药肿瘤细胞中也有此现象。在rHu-TNF敏感肿瘤细胞中，增强程度与对rHu-TNF的敏感性之间没有关系。当肿瘤细胞在接触rHu-TNF之前或接触rHu-TNF的早期用rHu-IFN-γ处理时，增强作用明显。另一方面，当肿瘤细胞在接触rHu-TNF的后期用rHu-IFN-γ处理时，未观察到增强作用。根据这些结果，预计两种药物联合治疗有望在临床试验中取得更好的效果。
