Inagami T, Iwai N, Sasaki K, Yamano Y, Bardhan S, Chaki S, Guo D F, Furuta H, Ohyama K, Kambayashi Y
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146.
Eur Heart J. 1994 Dec;15 Suppl D:104-7. doi: 10.1093/eurheartj/15.suppl_d.104.
Angiotensin II isoform 1 (AT1) receptor cDNAs were cloned by expression cloning from bovine adrenal and rat vascular smooth muscles. Human AT1 receptor was also cloned. Seven transmembrane structures emerged. A single type of receptor seems to interact with more than one type of G-protein. AT1 consists of subtypes AT1A and AT1B, and the regulation of the receptors occurs at many stages. The isoform AT2 was also expression cloned from rat pheochromocytoma cells. Although its ligand binding is not affected by GTP analogs, it is a seven transmembrane domain receptor. It mediates the inhibition of phosphotyrosine phosphatase by angiotensin II and AT2 specific CGP42112A; the inhibition was abolished by pertussis toxin. Thus, AT2 belongs to a new class of angiotensin receptors with unique signalling and regulatory mechanisms.
血管紧张素 II 同工型 1（AT1）受体的互补 DNA（cDNA）是通过从牛肾上腺和大鼠血管平滑肌进行表达克隆而获得的。人类 AT1 受体也被克隆出来。发现了七个跨膜结构。单一类型的受体似乎能与不止一种类型的 G 蛋白相互作用。AT1 由 AT1A 和 AT1B 亚型组成，并且受体的调节发生在多个阶段。同工型 AT2 也是从大鼠嗜铬细胞瘤细胞中通过表达克隆获得的。尽管其配体结合不受 GTP 类似物影响，但它是一种七跨膜结构域受体。它介导血管紧张素 II 和 AT2 特异性的 CGP42112A 对磷酸酪氨酸磷酸酶的抑制作用；百日咳毒素可消除这种抑制作用。因此，AT2 属于一类具有独特信号传导和调节机制的新型血管紧张素受体。
