Gancedo B, Alonso-Gómez A L, de Pedro N, Corpas I, Delgado M J, Alonso-Bedate M
Departamento de Biología Animal II (Fisiología Animal), Facultad de Ciencias Biológicas, Universidad Complutense, Madrid, Spain.
Gen Comp Endocrinol. 1995 Jan;97(1):66-75. doi: 10.1006/gcen.1995.1007.
Plasma T3 and T4 levels, and thyroid free (f) and bound (b) thyroid hormones contents, were determined by radioimmunoassay in adult male and female Rana perezi over 1 year. Plasma T3 levels show a significant seasonal cycle in both male and female frogs, increasing from January to peak in July (71-74 pg/ml) to decrease in August-October. Plasma T4 concentrations also varied seasonally, and sex differences were apparent. Basal T4 levels from the end of summer to the end of winter and higher values (682-651 pg/ml) in spring and early summer occur in female frogs; males have lowest levels in October (59.78 +/- 13.92 pg/ml) and higher levels during the rest of the year, except for a significant decrease in April. The thyroid content of fT3 and fT4 displayed similar seasonal patterns, with peak values in April and July-August. Sex differences are again present. The thyroid contents of bT3 are high between March and July in both sexes, with values in females being greater than those in males. The bT4 content follows a similar seasonal pattern in males and females and shows a minimum in March and a maximum in summer. Thyroid releasing capacity seems to change depending on the season and sex.
通过放射免疫分析法对成年雄性和雌性泽陆蛙进行了为期1年的血浆T3和T4水平以及甲状腺游离（f）和结合（b）甲状腺激素含量的测定。雄性和雌性青蛙的血浆T3水平均呈现出显著的季节性周期，从1月开始上升，在7月达到峰值（71 - 74 pg/ml），然后在8 - 10月下降。血浆T4浓度也随季节变化，且存在明显的性别差异。雌性青蛙从夏末到冬末的基础T4水平较低，而在春季和初夏则出现较高值（682 - 651 pg/ml）；雄性青蛙在10月T4水平最低（59.78 +/- 13.92 pg/ml），除4月显著下降外，一年中其余时间T4水平较高。游离T3和游离T4的甲状腺含量呈现相似的季节性模式，在4月和7 - 8月达到峰值。性别差异再次出现。结合T3的甲状腺含量在3月至7月期间两性均较高，雌性的值高于雄性。结合T4含量在雄性和雌性中呈现相似的季节性模式，在3月达到最低值，在夏季达到最高值。甲状腺释放能力似乎随季节和性别而变化。
