Iwasaki M, Nakahara H, Nakata K, Nakase T, Kimura T, Ono K
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
J Bone Joint Surg Am. 1995 Apr;77(4):543-54. doi: 10.2106/00004623-199504000-00007.
We studied the effects of transforming growth factor-beta and basic fibroblast growth factor on the regulation of proliferation and osteochondrogenic differentiation of periosteum-derived cells, which have the potential to differentiate into bone and hypertrophic cartilage in vitro. Histological observation revealed that transforming growth factor-beta stimulated chondrogenesis of periosteum-derived cells while basic fibroblast growth factor stimulated proliferation of fibroblast-like cells and inhibited osteochondrogenic differentiation. Immunohistochemical studies revealed that basic fibroblast growth factor inhibited the expression of osteocalcin. Transforming growth factor-beta enhanced uronic acid content but decreased DNA content, alkaline phosphatase activity, and calcium content. In contrast, basic fibroblast growth factor enhanced DNA content but decreased alkaline phosphatase activity, calcium content, and uronic acid content. In addition, transforming growth factor-beta shortened the time-course of gene expression of type-X collagen whereas basic fibroblast growth factor inhibited the gene expression. These results indicate that transforming growth factor-beta stimulates osteochondrogenic differentiation of periosteum-derived cells but inhibits proliferation. They also indicate that basic fibroblast growth factor stimulates proliferation of periosteum-derived cells but inhibits osteochondrogenic differentiation.
我们研究了转化生长因子-β和碱性成纤维细胞生长因子对骨膜来源细胞增殖及骨软骨生成分化调控的影响，这些细胞在体外具有分化为骨和肥大软骨的潜力。组织学观察显示，转化生长因子-β刺激骨膜来源细胞的软骨生成，而碱性成纤维细胞生长因子刺激成纤维样细胞增殖并抑制骨软骨生成分化。免疫组织化学研究显示，碱性成纤维细胞生长因子抑制骨钙素的表达。转化生长因子-β增加了糖醛酸含量，但降低了DNA含量、碱性磷酸酶活性和钙含量。相反，碱性成纤维细胞生长因子增加了DNA含量，但降低了碱性磷酸酶活性、钙含量和糖醛酸含量。此外，转化生长因子-β缩短了X型胶原基因表达的时间进程，而碱性成纤维细胞生长因子抑制该基因表达。这些结果表明，转化生长因子-β刺激骨膜来源细胞的骨软骨生成分化，但抑制增殖。它们还表明，碱性成纤维细胞生长因子刺激骨膜来源细胞增殖，但抑制骨软骨生成分化。
