Iwasaki M, Nakahara H, Nakase T, Kimura T, Takaoka K, Caplan A I, Ono K
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
J Bone Miner Res. 1994 Aug;9(8):1195-204. doi: 10.1002/jbmr.5650090809.
The effects of recombinant human bone morphogenetic protein 2 (rhBMP-2) on osteochondrogenesis were examined in high-density cultures of periosteum-derived cells, which have the potential to differentiate into bone and hypertrophic cartilage in vitro. Proliferation of these cells was inhibited by treatment with rhBMP-2. The time course for alkaline phosphatase (ALP) expression was shortened and the mineralization of the culture was increased by supplementation with rhBMP-2. These stimulatory effects of rhBMP-2 were observed at doses of 10-100 ng/m. Bone Gla protein (BGP) was immunocytochemically detectable earlier in the culture treated with rhBMP-2, and the BGP-positive layer of the rhBMP-2-treated cultures was thicker than that of the control cultures. On the other hand, there was no difference in uronic acid content or the time course of alpha 1(II) collagen mRNA expression between the rhBMP-2-treated and the control cultures. These results indicate that rhBMP-2 shortens the time course of osteogenesis and increases the amount of bone formation, whereas chondrogenesis remains unaffected.
在骨膜来源细胞的高密度培养中检测了重组人骨形态发生蛋白2（rhBMP-2）对骨软骨生成的影响，该细胞在体外具有分化为骨和肥大软骨的潜力。用rhBMP-2处理可抑制这些细胞的增殖。补充rhBMP-2可缩短碱性磷酸酶（ALP）表达的时间进程，并增加培养物的矿化。在10-100 ng/m的剂量下观察到rhBMP-2的这些刺激作用。在用rhBMP-2处理的培养物中，骨钙蛋白（BGP）在免疫细胞化学上可更早检测到，且rhBMP-2处理培养物的BGP阳性层比对照培养物的更厚。另一方面，rhBMP-2处理的培养物与对照培养物之间在糖醛酸含量或α1（II）型胶原mRNA表达的时间进程上没有差异。这些结果表明，rhBMP-2缩短了成骨的时间进程并增加了骨形成量，而软骨生成不受影响。
