Jardi R, Buti M, Rodriguez F, Cotrina M, Allende H, Esteban R, Guardia J
Department of Biochemistry, Hospital General Valle Hebrón, Universidad Autónoma, Barcelona, Spain.
J Virol Methods. 1994 Dec;50(1-3):59-66. doi: 10.1016/0166-0934(94)90163-5.
To study the concordance, sensitivity and specificity of HDV-RNA determination by molecular hybridization, serum HDAg by immunoblot and anti-HD IgM by commercial enzyme immunoassay as compared to intrahepatic HDAg detection by an immunoperoxidase method, a statistical analysis was applied to the results of serum sample and liver biopsy determinations in 50 patients with chronic delta hepatitis (38 positive to tissue HDAg and 12 negative). Of the 38 patients with hepatic HDAg, HDV-RNA was found in 31 (82%), serum HDAg by immunoblot in 27 (71%) and anti-HD IgM in 33 (87%). Among the 12 patients without hepatic HDAg, one was found with serum HDAg using the immunoblot technique, two (17%) had HDV-RNA, and 7 (58%) had anti-HD IgM. Serum HDAg determination by immunoblot was the most specific test, followed by HDV-RNA analysis. The least specific was the anti-HD IgM technique. The anti-HD IgM test was the most sensitive, followed by HDV-RNA and serum HDAg. The concordance with intrahepatic HDAg detection was highest for HDV-RNA determination, followed by HDAg in serum. The least degree of concordance was found with anti-HD IgM determination. These results suggest that the determination of HDV-RNA by the hybridization method can be of great value for the diagnosis and monitoring of chronic delta hepatitis.
为研究分子杂交法检测HDV-RNA、免疫印迹法检测血清HDAg以及商业酶免疫法检测抗-HD IgM与免疫过氧化物酶法检测肝内HDAg的一致性、敏感性和特异性，对50例慢性丁型肝炎患者（38例肝组织HDAg阳性，12例阴性）的血清样本和肝活检检测结果进行了统计分析。在38例肝组织HDAg阳性患者中，31例（82%）检测到HDV-RNA，27例（71%）免疫印迹法检测到血清HDAg，33例（87%）检测到抗-HD IgM。在12例肝组织HDAg阴性患者中，1例采用免疫印迹技术检测到血清HDAg，2例（17%）检测到HDV-RNA，7例（58%）检测到抗-HD IgM。免疫印迹法检测血清HDAg是最具特异性的检测方法，其次是HDV-RNA分析。特异性最低的是抗-HD IgM技术。抗-HD IgM检测是最敏感的，其次是HDV-RNA和血清HDAg。HDV-RNA检测与肝内HDAg检测的一致性最高，其次是血清HDAg。抗-HD IgM检测的一致性程度最低。这些结果表明，杂交法检测HDV-RNA对慢性丁型肝炎的诊断和监测具有重要价值。
