Demir A, Alfthan H, Stenman U H, Voutilainen R
Department of Pediatrics, Helsinki University Central Hospital, Finland.
Pediatr Res. 1994 Aug;36(2):221-6. doi: 10.1203/00006450-199408000-00014.
To study the feasibility of noninvasive sampling in pediatric patients, we examined the concentrations of LH and FSH in paired serum and urine samples from 65 children (age 0-15 y) with highly sensitive time-resolved immunofluorometric assays. The detection limits of the assays were 0.015 IU/L for LH and 0.018 IU/L for FSH. These sensitivity levels allowed quantification of the low prepubertal LH and FSH concentrations. The correlation between serum and urine gonadotropin values was very good (r = 0.751, p < 0.001 for FSH; and r = 0.720, p < 0.001 for LH), and the urine and serum concentrations were very similar. Correction of urinary gonadotropin concentrations for changes in urinary flow by standard methods using density [concentration x (0.02/density-1)] or creatinine (concentration/creatinine) did not improve the correlation. Therefore, measurement of urinary gonadotropins without correction can simply be used in the pediatric outpatient setting as a noninvasive alternative to serum determinations.
为研究儿科患者无创采样的可行性，我们采用高灵敏度时间分辨免疫荧光分析法检测了65名0至15岁儿童配对的血清和尿液样本中促黄体生成素（LH）和促卵泡生成素（FSH）的浓度。该检测方法对LH的检测限为0.015 IU/L，对FSH的检测限为0.018 IU/L。这些灵敏度水平能够对青春期前较低的LH和FSH浓度进行定量分析。血清和尿液中促性腺激素值之间的相关性非常好（FSH：r = 0.751，p < 0.001；LH：r = 0.720，p < 0.001），且尿液和血清浓度非常相似。使用密度[浓度×(0.02/密度 - 1)]或肌酐（浓度/肌酐）的标准方法对尿流量变化进行校正后，并未改善促性腺激素浓度的相关性。因此，在儿科门诊环境中，无需校正即可测量尿促性腺激素，作为血清检测的无创替代方法。
