Katoch V M
CJIL Field Unit (ICMR), Madras.
Indian J Lepr. 1994 Oct-Dec;66(4):455-62.
Morphological characteristics have been used as a parameter to assess the viability of M.leprae in leprosy patients. However, with the advent of the mouse foot-pad technique, viability of M.leprae is determined by growing the bacilli in the mouse foot-pad. In recent years, a fluorescent staining technique using fluorescent diacetate-ethidium bromide (FDA-EB) has been used to assess the viability of cultivable mycobacteria as well as M.leprae. The purpose of this study was to compare the viability of M.leprae by both mouse foot-pad and fluorescent staining techniques. M.leprae strains from both untreated and treated patients as well as mouse passaged strains of M.leprae were used for the comparison. Percentage of green-stained bacilli in the inoculum was compared with that of multiplication of M.leprae in the mouse foot-pad. It was observed that there was no correlation between the estimates of viable M.leprae by fluorescent staining and by mouse foot-pad inoculation. FDA-EB staining appears to reflect only trends as absence of green staining cells had overall general correlation with loss of infectivity to mouse foot-pad but, the converse was not found to be true.
形态学特征已被用作评估麻风病人中麻风杆菌活力的一个参数。然而，随着小鼠足垫技术的出现，麻风杆菌的活力是通过在小鼠足垫中培养杆菌来确定的。近年来，一种使用荧光双醋酸盐-溴化乙锭（FDA-EB）的荧光染色技术已被用于评估可培养分枝杆菌以及麻风杆菌的活力。本研究的目的是通过小鼠足垫和荧光染色技术比较麻风杆菌的活力。来自未治疗和已治疗患者的麻风杆菌菌株以及小鼠传代的麻风杆菌菌株用于该比较。将接种物中绿色染色杆菌的百分比与麻风杆菌在小鼠足垫中的增殖情况进行比较。观察到通过荧光染色和小鼠足垫接种对活麻风杆菌的估计之间没有相关性。FDA-EB染色似乎仅反映趋势，因为没有绿色染色细胞总体上与对小鼠足垫感染力的丧失有普遍相关性，但反之则不成立。
