Mercer A R, Hayashi J H, Hildebrand J G
Arizona Research Laboratories, Division of Neurobiology, University of Arizona, Tucson 85721.
J Exp Biol. 1995 Mar;198(Pt 3):613-27. doi: 10.1242/jeb.198.3.613.
The modulatory effects of 5-hydroxytryptamine (5-HT or serotonin) on voltage-gated currents in central olfactory neurones of the moth Manduca sexta have been examined in vitro using whole-cell patch-clamp recording techniques. Central olfactory neurones were dissociated from the antennal lobes of animals at stage 5 of the 18 stages of metamorphic adult development. The modulatory actions of 5-HT on voltage-activated ionic currents were examined in a subset of morphologically identifiable antennal lobe neurones maintained for 2 weeks in primary cell culture. 5-HT caused reversible reduction of both a rapidly activating A-type K+ current and a relatively slowly activating K+ current resembling a delayed rectifier-type conductance. 5-HT also reduced the magnitude of voltage-activated Ca2+ influx in these cells. The functional significance of 5-HT-modulation of central neurones is discussed.
利用全细胞膜片钳记录技术，在体外研究了5-羟色胺（5-HT或血清素）对烟草天蛾中枢嗅觉神经元电压门控电流的调节作用。中枢嗅觉神经元取自变态发育成虫18个阶段中第5阶段动物的触角叶。在原代细胞培养中维持2周的一部分形态可识别的触角叶神经元中，研究了5-HT对电压激活离子电流的调节作用。5-HT可使快速激活的A型钾电流和类似于延迟整流型电导的相对缓慢激活的钾电流均发生可逆性降低。5-HT还降低了这些细胞中电压激活的钙离子内流幅度。文中讨论了5-HT对中枢神经元调节的功能意义。
