Mercer A R, Kirchhof B S, Hildebrand J G
Arizona Research Laborator, University of Arizona, Tucson 85721, USA.
J Neurobiol. 1996 Jan;29(1):49-64. doi: 10.1002/(SICI)1097-4695(199601)29:1<49::AID-NEU4>3.0.CO;2-7.
Cell culture experiments have been used to examine the effects of serotonin [5-hydroxytryptamine (5-HT)] on the morphological development of antennal lobe (AL) neurons in the brain of the sphinx moth, Manduca sexta. The majority of cells used in this study were from animals at stage 5 of the 18 stages of metamorphic adult development. 5-HT did not affect the survival of M. sexta AL neurons in culture, but did increase the numbers of cells displaying features characteristic of certain cell types. Three morphologically distinct cell types were examined in detail. The principal effect of 5-HT on these neurons was enhancement of cell growth. The magnitude of responses to this amine was cell-type specific. Site-specific responses to 5-HT were apparent also in one cell type. Our results suggest that the effects of 5-HT can change during the course of metamorphic development. These changes coincide temporally with the development of fast, sodium-based action potentials.
细胞培养实验已被用于研究血清素[5-羟色胺（5-HT）]对烟草天蛾大脑触角叶（AL）神经元形态发育的影响。本研究中使用的大多数细胞来自变态成虫发育18个阶段中的第5阶段的动物。5-HT不影响培养的烟草天蛾AL神经元的存活，但确实增加了显示某些细胞类型特征的细胞数量。详细研究了三种形态不同的细胞类型。5-HT对这些神经元的主要作用是促进细胞生长。对这种胺的反应程度具有细胞类型特异性。在一种细胞类型中也明显存在对5-HT的位点特异性反应。我们的结果表明，5-HT的作用可能在变态发育过程中发生变化。这些变化在时间上与快速的、基于钠的动作电位的发育相吻合。
