Pollack G S, Lakes-Harlan R
Department of Biology, McGill University, Montréal, Québec, Canada.
J Neurobiol. 1995 Jan;26(1):17-32. doi: 10.1002/neu.480260103.
We studied the birth times of neurons of labellar taste sensilla in blowflies using incorporation of the thymidine analogue 5-bromodeoxyuridine (BrdU) as an indicator of birth time. We found that one of the two main sensillum types, the taste papillae, arise according to a clear spatial gradient of birth times, whereas the other sensillum type, taste hairs, arise without any apparent spatial ordering. Within each sensillum type, there was a strong tendency for either all or none of the neurons to have incorporated BrdU. Among those rare sensilla in which only some of the neurons incorporated BrdU, there were clear patterns of the distribution of labeled and unlabeled neurons per sensillum. These results suggest that subsets of the neurons of a sensillum are siblings, and thus argue against the possibility that the several neurons of a sensillum arise from a single stem cell precursor through repeated asymmetrical divisions.
我们使用胸腺嘧啶类似物5-溴脱氧尿苷（BrdU）掺入法作为出生时间的指标，研究了丽蝇唇叶味觉感器神经元的出生时间。我们发现，两种主要感器类型之一，即味觉乳突，按照明确的出生时间空间梯度产生，而另一种感器类型，即味觉毛，则没有任何明显的空间排序。在每种感器类型中，存在一种强烈的趋势，即所有神经元要么都掺入了BrdU，要么都没有掺入。在那些只有部分神经元掺入BrdU的罕见感器中，每个感器中标记和未标记神经元的分布有明显的模式。这些结果表明，一个感器的神经元亚群是同胞神经元，因此反对一个感器的几个神经元通过反复不对称分裂从单个干细胞前体产生的可能性。
