Ray K, Hartenstein V, Rodrigues V
Molecular Biology Unit, Tata Institute of Fundamental Research, Bombay, India.
Dev Biol. 1993 Jan;155(1):26-37. doi: 10.1006/dbio.1993.1003.
The taste bristles of the adult labellum develop from the labial discs within the first 30 hr of pupation. The neuron-specific antibody Mab22C10 and the tissue-specific beta-galactosidase activity in the A37 strain were used as cell markers for the developing sensilla. These experiments revealed that the sensory progenitors of all the labellar bristles are specified in three waves occurring 0, 6, and 16 hr after pupation. The incorporation of the thymidine analog 5-bromo-2'-deoxyuridine (BrdU) into the cells of the developing proboscis further supports the temporal pattern of the sensillum specification. Mitotic activity in the bristles specified in the first wave is completed before the next wave is initiated. The eight cells that constitute a single bristle share a common lineage. The trichogen and tormogen cells are siblings from a single progenitor and the thecogen and the five neurons share a common ancestry. Our results provide a basis for the identification of molecules that specify the development of the polyinnervated bristles of the adult and the specification of neurons with different stimulus specificities.
成虫唇叶的味觉刚毛在化蛹后的前30小时内从唇盘发育而来。神经元特异性抗体Mab22C10和A37品系中的组织特异性β-半乳糖苷酶活性被用作发育中感觉器的细胞标记。这些实验表明，所有唇叶刚毛的感觉祖细胞在化蛹后0、6和16小时分三波确定。将胸苷类似物5-溴-2'-脱氧尿苷（BrdU）掺入发育中喙的细胞中进一步支持了感觉器确定的时间模式。第一波确定的刚毛中的有丝分裂活动在下一波开始之前完成。构成单个刚毛的八个细胞具有共同的谱系。毛原细胞和膜原细胞是来自单个祖细胞的姐妹细胞，鞘原细胞和五个神经元具有共同的祖先。我们的结果为鉴定决定成虫多神经支配刚毛发育的分子以及具有不同刺激特异性的神经元的确定提供了基础。
