Yokota M, Peterson J W, Kaoutzanis M C, Yamakawa K, Sibilia R, Zervas N T
Neurological Service, Massachusetts General Hospital, Boston, USA.
J Neurosurg. 1995 May;82(5):834-40. doi: 10.3171/jns.1995.82.5.0834.
Sixteen dogs were entered into a study of the double subarachnoid hemorrhage (SAH) model of cerebral vasospasm. Six animals were sacrificed 72 hours after the first experimental SAH, and the remaining 10 animals were killed 72 hours after the second experimental SAH; ten additional animals served as controls. Basilar arteries were rapidly excised from the dogs and frozen. Multiple segments of the frozen arteries were analyzed independently for total protein and 1,2-diacylglycerol (DAG) content, which averaged 3.17 (+/- 0.27 standard error of the mean; SEM) pmol DAG/microgram protein for all 25 arteries analyzed. A slight decreasing trend in DAG content relative to that of control vessels was found in vessels chronically constricted in situ by subarachnoid blood clot; however, this trend did not attain statistical significance. Two segments of the same vessels were assayed independently for protein kinase C (PKC) activity, which averaged 1.21 (+/- 0.08 SEM) pmol phosphate incorporation per minute per microgram protein for all 24 arteries analyzed. A small decrease in PKC content was noted in vessels that experienced a single SAH; however, PKC returned to near control value in vessels subjected to double SAH. The ratio of particulate (membrane bound) to soluble PKC activity, an indicator of PKC translocation to the membrane and hence PKC activation, showed a small but statistically significant trend to increase with experimental SAH.
16只狗被纳入一项关于脑血管痉挛双蛛网膜下腔出血（SAH）模型的研究。6只动物在首次实验性SAH后72小时处死后，其余10只动物在第二次实验性SAH后72小时处死；另外10只动物作为对照。迅速从狗身上取出基底动脉并冷冻。对冷冻动脉的多个节段独立分析总蛋白和1,2 - 二酰甘油（DAG）含量，在所分析的全部25条动脉中，平均为3.17（±0.27平均标准误；SEM）pmol DAG/微克蛋白。在因蛛网膜下腔血凝块而在原位长期收缩的血管中，发现DAG含量相对于对照血管有轻微下降趋势；然而，这一趋势未达到统计学显著性。对同一些血管的两个节段独立测定蛋白激酶C（PKC）活性，在所分析的全部24条动脉中，平均为1.21（±0.08 SEM）pmol磷酸盐掺入量每分钟每微克蛋白。在经历单次SAH的血管中，注意到PKC含量有小幅下降；然而，在经历双次SAH的血管中，PKC恢复到接近对照值。颗粒状（膜结合）与可溶性PKC活性的比值，作为PKC向膜转位进而PKC激活的一个指标，显示出随实验性SAH有小幅但具有统计学显著性的增加趋势。
