Yamakawa K, Peterson J W, Sibilia R, Zervas N T
Neurosurgical Service, Massachusetts General Hospital, Boston, USA.
Neurosurgery. 1995 Apr;36(4):789-96. doi: 10.1227/00006123-199504000-00021.
A causal or supportive relationship between 1,2-diacylglycerol (DAG) content and the maintenance of tonic vasoconstriction was sought in canine basilar arteries treated in vitro with various agents reported to increase DAG levels in other tissues (platelet-derived growth factor, vasopressin, angiotensin II, and endothelin-1) and, conversely, with agents known to activate sustained constriction (high K+, phorbol ester, hemolysate, and endothelin-1). Multiple segments from individual isolated arteries were prepared. Some segments were immediately frozen as controls and others incubated in physiological saline solution at 37 degrees C for either 5 minutes or 30 minutes in the presence or absence of different concentrations of the test materials. Segments were then quickly frozen until homogenized for lipid extraction and DAG assay. The DAG content of samples incubated up to 2 hours in physiological saline solution alone did not significantly differ from that of immediately frozen control samples. Resting DAG content expressed relative to total protein measured in each sample averaged 3.82 +/- 0.26 (standard error of the mean) pmol DAG/microgram of protein (74 samples from 37 arteries). Endothelin at 2 x 10(-7) mol/L led to a statistically significant increase in DAG content of approximately 40% of basal content at 5 and 30 minutes. A smaller increase in DAG attributable to hemolysate (approximately 25%) was statistically significant at 30 minutes, whereas vasopressin provoked a notable decrease in DAG content. The other agents had no effect. No differences in these results were noted between normal canine basilar arteries and arteries constricted in vivo by subarachnoid blood clot before isolation.(ABSTRACT TRUNCATED AT 250 WORDS)
在体外，用据报道能增加其他组织（血小板衍生生长因子、血管加压素、血管紧张素 II 和内皮素 -1）中 1,2 - 二酰甘油（DAG）水平的各种药剂，以及相反地，用已知能激活持续性收缩的药剂（高钾、佛波酯、溶血产物和内皮素 -1）处理犬基底动脉，探寻 DAG 含量与紧张性血管收缩维持之间的因果或支持关系。从单个分离的动脉制备多个节段。一些节段立即冷冻作为对照，其他节段在 37℃的生理盐溶液中，在有或没有不同浓度测试材料的情况下孵育 5 分钟或 30 分钟。然后将节段快速冷冻直至匀浆以进行脂质提取和 DAG 测定。仅在生理盐溶液中孵育长达 2 小时的样品的 DAG 含量与立即冷冻的对照样品的 DAG 含量无显著差异。相对于每个样品中测量的总蛋白表示的静息 DAG 含量平均为 3.82±0.26（平均值的标准误差）pmol DAG/微克蛋白（来自 37 条动脉的 74 个样品）。2×10⁻⁷mol/L 的内皮素在 5 分钟和 30 分钟时导致 DAG 含量统计学上显著增加，约为基础含量的 40%。溶血产物引起的 DAG 较小增加（约 25%）在 30 分钟时具有统计学意义，而血管加压素引起 DAG 含量显著下降。其他药剂无作用。在正常犬基底动脉和分离前在体内因蛛网膜下腔血凝块而收缩的动脉之间，未观察到这些结果的差异。（摘要截短于 250 字）
