Cheung P K, Visser J, Bakker W W
Department of Pathology, University of Groningen, The Netherlands.
J Pharm Pharmacol. 1994 Dec;46(12):1032-4. doi: 10.1111/j.2042-7158.1994.tb03262.x.
Ecto ATP-diphosphohydrolase (apyrase) activity of human endothelial cells following aspirin treatment has been studied in-vitro. It was shown by HPLC analysis of supernatant samples that pre-incubation of the cultures with aspirin resulted in a significantly increased turnover of supplemented ATP into its degradation products (ADP and AMP). Enhanced expression of ectoenzyme after aspirin treatment could be observed as demonstrated by immunofluorescence-staining with monoclonal anti-apyrase antibodies. This suggests enhancement of endothelial ATP-diphosphohydrolase activity induced by aspirin. The present data obtained in human vascular cells in-vitro are in line with results from previous animal studies in-vivo, suggesting a novel cyclooxygenase-independent antithrombotic activity of aspirin.
已在体外研究了阿司匹林处理后人内皮细胞的ecto ATP-二磷酸水解酶（腺苷三磷酸双磷酸酶）活性。通过对上清液样品的高效液相色谱分析表明，用阿司匹林预孵育培养物会导致补充的ATP向其降解产物（ADP和AMP）的周转率显著增加。如用单克隆抗腺苷三磷酸双磷酸酶抗体进行免疫荧光染色所示，可观察到阿司匹林处理后胞外酶的表达增强。这表明阿司匹林诱导内皮ATP-二磷酸水解酶活性增强。目前在人血管细胞体外获得的数据与先前动物体内研究的结果一致，提示阿司匹林具有一种新的不依赖环氧化酶的抗血栓活性。
