Mackie P D, Zhang H Q, Schmidt R F, Rowe M J
School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia.
J Neurosci Methods. 1995 Jan;56(1):31-5. doi: 10.1016/0165-0270(94)00086-v.
A preparation is described that permits the monitoring of activity from individual joint afferent nerve fibres in an intact peripheral nerve of the cat. The joint nerve used was the medial articular nerve (MAN) that supplies the medial and anteromedial aspects of the knee joint. This nerve is sufficiently fine that if freed from nearby tissue over a length of 2-5 cm and placed over a platinum hook electrode it is possible to identify and monitor, from the intact nerve, the impulse activity from each group II joint afferent fibre activated by mechanical stimulation of the joint capsule. The signal-to-noise ratio exceeds 5:1 and in most cases was approximately 10:1. With this preparation it is now possible to examine the central actions and security of transmission at central synaptic targets for single, identified group II joint afferent fibres.
本文描述了一种可用于监测猫完整外周神经中单个关节传入神经纤维活动的制备方法。所使用的关节神经是供应膝关节内侧和前内侧的内侧关节神经（MAN）。该神经足够细，若在2 - 5厘米的长度范围内从附近组织游离出来并置于铂钩电极上，就有可能从完整神经中识别并监测由关节囊机械刺激激活的每组II类关节传入纤维的冲动活动。信噪比超过5:1，在大多数情况下约为10:1。利用这种制备方法，现在可以研究单个、已识别的II类关节传入纤维在中枢突触靶点的中枢作用和传递安全性。
