Mahns David A, Ivanusic Jason J, Sahai Vineet, Rowe Mark J
Department of Physiology and Pharmacology, School of Medical Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
J Neurosci Methods. 2006 Sep 30;156(1-2):140-4. doi: 10.1016/j.jneumeth.2006.02.019. Epub 2006 Mar 30.
A preparation is described in which it is possible to selectively activate and monitor the activity of the individual periosteal afferent nerve fibres arising from the humerus bone of the cat. The nerve is a fine branch of the median nerve that accompanies the small artery and vein that enter the nutrient foramen of the humerus. By freeing this fine nerve from nearby tissue over a length of approximately 1-2 cm and placing it over a silver hook recording electrode, it becomes possible to identify and monitor electrophysiologically, the impulse activity of individual periosteal afferent fibres activated by focal mechanical stimulation of the periosteum. With this preparation it will be possible to examine the central actions and security of transmission at central synaptic targets for single, small-diameter afferent fibres arising from bone.
本文描述了一种制备方法，利用该方法可以选择性激活并监测源自猫肱骨的单个骨膜传入神经纤维的活性。该神经是正中神经的一个细分支，与进入肱骨滋养孔的小动脉和小静脉相伴行。通过在大约1 - 2厘米的长度范围内将这条细神经从附近组织中游离出来，并将其置于银钩记录电极上，就能够通过电生理学方法识别和监测因骨膜局部机械刺激而激活的单个骨膜传入纤维的冲动活性。利用这种制备方法，将有可能研究源自骨骼的单个小直径传入纤维在中枢突触靶点处的中枢作用和传递安全性。
