Vo-Dinh T, Panjehpour M, Overholt B F, Farris C, Buckley F P, Sneed R
Oak Ridge National Laboratory, Tennessee 37831, USA.
Lasers Surg Med. 1995;16(1):41-7. doi: 10.1002/lsm.1900160106.
We report the use of new diagnostic parameters based on the differential normalized fluorescence (DNF) signals for malignant tumor diagnosis.
STUDY DESIGN/MATERIALS AND METHODS: Over 200 measurements of endogenous fluorescence from normal and malignant esophageal tissues were performed during routine endoscopy in 48 patients. A pulsed nitrogen-pumped dye laser was used to provide in situ excitation at 410 nm. Direct collection of the fluorescence signal emitted by the tissue was achieved using an intensified photodiode array detector equipped with a fiberoptic probe.
The fluorescence signals were normalized with respect to the total fluorescence signal area. The cancer diagnosis indices were defined by the difference between the normalized fluorescence signal of a tumor and the mean value of a reference set of normal tissues. The results of the DNF approach were compared with endoscopic examinations and histopathology interpretations of the biopsy samples. Excellent correlation in the classification of normal and malignant tumors for the samples was found.
The data indicated that the DNF approach has a significant potential to provide a direct, real-time, and in-situ technique for cancer diagnosis of the esophagus without requiring biopsy of the tumors and time-consuming histopathology tests.
我们报告了基于差异归一化荧光（DNF）信号的新诊断参数在恶性肿瘤诊断中的应用。
研究设计/材料与方法：在48例患者的常规内镜检查过程中，对正常和恶性食管组织的内源性荧光进行了200多次测量。使用脉冲氮泵浦染料激光器在410nm处进行原位激发。通过配备光纤探头的增强型光电二极管阵列探测器直接收集组织发出的荧光信号。
荧光信号相对于总荧光信号面积进行归一化。癌症诊断指标由肿瘤归一化荧光信号与正常组织参考集平均值之间的差异定义。将DNF方法的结果与活检样本的内镜检查和组织病理学解释进行了比较。发现样本在正常和恶性肿瘤分类方面具有良好的相关性。
数据表明，DNF方法具有显著潜力，可为食管癌诊断提供一种直接、实时且原位的技术，无需对肿瘤进行活检和耗时的组织病理学检测。
