一种RNA聚合酶II和III转录人snRNA基因所需的TBP-TAF复合物。
A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III.
作者信息
Henry R W, Sadowski C L, Kobayashi R, Hernandez N
机构信息
Cold Spring Harbor Laboratory, New York 11724, USA.
出版信息
Nature. 1995 Apr 13;374(6523):653-6. doi: 10.1038/374653a0.
The TATA-box-binding protein TBP exists in the cell complexed with different sets of TBP-associated factors (TAFs). In general, each of these TBP-TAF complexes is dedicated to transcription by a single RNA polymerase. Thus, SL1, TFIID and TFIIIB are required for transcription by polymerases I, II and III, respectively. Here we characterize a fourth TBP-TAF complex called SNAPc. Unlike the other TBP-TAF complexes, SNAPc is implicated in transcription by two types of polymerases; it is required for transcription of both the RNA polymerase II and III small-nuclear RNA genes and binds specifically to the proximal sequence element PSE, a non-TATA-box basal promoter element common to these two types of genes. In addition to TBP, SNAPc is composed of at least three TAFs, SNAP43, SNAP45 and SNAP50. The predicted amino-acid sequence of SNAP43 reveals that it corresponds to a new protein.
TATA 框结合蛋白 TBP 在细胞中与不同的 TBP 相关因子（TAF）结合形成复合物存在。一般来说，这些 TBP-TAF 复合物中的每一种都专门用于单一 RNA 聚合酶的转录。因此，SL1、TFIID 和 TFIIIB 分别是聚合酶 I、II 和 III 转录所必需的。在这里，我们鉴定了一种名为 SNAPc 的第四个 TBP-TAF 复合物。与其他 TBP-TAF 复合物不同，SNAPc 参与两种类型聚合酶的转录；它是 RNA 聚合酶 II 和 III 小核 RNA 基因转录所必需的，并且特异性结合近端序列元件 PSE，这是这两种基因共有的非 TATA 框基础启动子元件。除了 TBP，SNAPc 至少由三种 TAF 组成，即 SNAP43、SNAP45 和 SNAP50。SNAP43 的预测氨基酸序列表明它对应一种新蛋白质。
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