SNAP19介导RNA聚合酶II和III共享的功能性核心启动子复合物（SNAPc）的组装。
SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III.
作者信息
Henry R W, Mittal V, Ma B, Kobayashi R, Hernandez N
机构信息
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
出版信息
Genes Dev. 1998 Sep 1;12(17):2664-72. doi: 10.1101/gad.12.17.2664.
Abstract
The basal transcription factor SNAPc binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAPc contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAPc. Here we reconstitute SNAPc from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAPc nucleates the assembly of two classes of initiation complexes.
摘要
基础转录因子SNAPc与PSE结合，PSE是RNA聚合酶II和III人类小核RNA启动子中的核心元件。SNAPc至少包含四个亚基，但尚未成功组装出完全确定的重组SNAPc。在这里，我们用五个重组亚基SNAP43、SNAP45、SNAP50、SNAP190和一个新鉴定的亚基SNAP19重构了SNAPc。这种重组复合物特异性地结合PSE，并指导RNA聚合酶II和III小核RNA基因的转录。因此，同一个核心SNAPc促成了两类起始复合物的组装。
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